ELISA Kit for Kidney Injury Molecule 1 (Kim1)

78-5, 000pg/mL

The minimum detectable dose of this kit is typically less than 32pg/mL

3h

This assay has high sensitivity and excellent specificity for detection of Kidney Injury Molecule 1 (Kim1).
No significant cross-reactivity or interference between Kidney Injury Molecule 1 (Kim1) and analogues was observed.

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Kidney Injury Molecule 1 (Kim1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Kidney Injury Molecule 1 (Kim1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Kidney Injury Molecule 1 (Kim1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Kidney Injury Molecule 1 (Kim1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Clinical implication of urinary neutrophil gelatinase-associated lipocalin and kidney injury molecule-1 in patients with acute paraquat intoxication; KIM-1 and NGAL: new markers of obstructive nephropathy; Urinary Neutrophil Gelatinase-Associated Lipocalin at Birth Predicts Early Renal Function in Very Low Birth Weight Infants; Markers of kidney function in the elderly in relation to the new CKD-EPI formula for estimation of glomerular filtration rate; Interleukin 18 as a Marker of Chronic Nephropathy in Children after Anticancer Treatment; Kidney injury molecule-1: a urinary biomarker for contrast induced acute kidney injury.; Urinary neutrophil gelatinase-associated lipocalin predicts graft loss after acute kidney injury in kidney transplant; 中性粒细胞明胶酶相关脂质运载蛋白、肾损伤分子1和白介素18在诊断心肺分流术后急性肾损伤中的意义; Tubular urinary biomarkers do not identify aetiology of acute kidney injury in kidney transplant recipients; SYNbiotics Easing Renal failure by improving Gut microbiologY (SYNERGY): a protocol of placebo-controlled randomised cross-over trial; The diagnosis of febrile urinary tract infection in children may be facilitated by urinary biomarkers; Kidney injury molecule-1: A urinary biomarker for contrast-induced acute kidney injury; Role of new biomarkers for predicting renal scarring in vesicoureteral reflux: NGAL, KIM-1, and L-FABP; Urinary NGAL, KIM-1 and L-FABP concentrations in antenatal hydronephrosis; Kidney Injury Molecule Levels in Type 2 Diabetes Mellitus; Urinary Kidney Injury Molecule-1 but Not Urinary Neutrophil Gelatinase Associated Lipocalin Is Increased after Short Maximal Exercise; Urinary neutrophil gelatinase-associated lipocalin, kidney injury molecule-1, N-acetyl-β-D-glucosaminidase levels and mortality risk in septic patients with …; The Kidney Specific Protein myo-Inositol Oxygenase, a Potential Biomarker for Diabetic Nephropathy; Urine markers of renal tubular injury in idiopathic membranous nephropathy: A cross sectional study; Application of ELISA Technique and Human Microsomes in the Search for 11β-Hydroxysteroid Dehydrogenase Inhibitors;