ELISA Kit for Procalcitonin (PCT)

31.2-2, 000pg/mL

The minimum detectable dose of this kit is typically less than 12.4pg/mL

3h

This assay has high sensitivity and excellent specificity for detection of Procalcitonin (PCT).
No significant cross-reactivity or interference between Procalcitonin (PCT) and analogues was observed.

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Procalcitonin (PCT). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Procalcitonin (PCT). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Procalcitonin (PCT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Procalcitonin (PCT) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Evaluation of neutrophilic CD64, interleukin 10 and procalcitonin as diagnostic markers of early- and late-onset neonatal sepsis; Effects of Berberine Against Radiation-Induced Intestinal Injury in Mice; Diagnostic and prognostic value of procalcitonin and phosphorus in acute mesenteric ischemia; Serum procalcitonin and its relation to the outcome of ventilator-associated pneumoniain children; The Assessment of Vitamin D, Antimicrobial Peptides and Procalcitonin in Bronchiectasis; Evaluation of the Diagnostic Utility of Fc Gamma Receptor Type 1 (nCD64) and Procalcitonin in Comparison with Conventional Methods for Diagnosis of Early Onset Neonatal Sepsis; Efficacy of measuring procalcitonin levels in determination of prognosis and early diagnosis of bacterial resistance in sepsis; The use and value of procalcitonin in solid organ transplantation; Macrocalcitonin is a novel pitfall in the routine of serum calcitonin immunoassay; Procalcitonin and Pentraxin-3: Current biomarkers in inflammation in white coat hypertension; Risk Factors of Acute Pancreatitis in Oral Double Balloon Enteroscopy.; Changes in inflammatory mediators as a result of intermittent hypoxia in obstructive sleep apnea syndrome; INTERLEUKIN-1 RECEPTOR 2: A NEW BIOMARKER FOR SEPSIS DIAGNOSIS AND GRAM-NEGATIVE/GRAM-POSITIVE BACTERIAL DIFFERENTIATION; Inflammatory mediators in the diagnosis and treatment of acute pancreatitis: pentraxin-3, procalcitonin and myeloperoxidase; Procalcitonin and C-reactive protein as peripheral inflammatory markers in antipsychotic drug-free schizophrenia patients;