ELISA Kit for Toll Like Receptor 2 (TLR2)

0.312-20ng/mL

The minimum detectable dose of this kit is typically less than 0.112ng/mL

3h

This assay has high sensitivity and excellent specificity for detection of Toll Like Receptor 2 (TLR2).
No significant cross-reactivity or interference between Toll Like Receptor 2 (TLR2) and analogues was observed.

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Toll Like Receptor 2 (TLR2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Toll Like Receptor 2 (TLR2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Toll Like Receptor 2 (TLR2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Toll Like Receptor 2 (TLR2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Expression of soluble Toll-like receptors in pleural effusions; TLR2 in Pleural Fluid Is Modulated by Talc Particles during Pleurodesis; The Possible Role of TLR2 in Chronic Hepatitis B Patients with Precore Mutation; Cervical and vaginal fluid soluble Toll-like receptor 2 in pregnancies complicated by preterm prelabor rupture of membranes; Cytokines and Toll-Like Receptors in the Immune Response to Influenza Vaccination; Correlation?between?salivary?Toll like receptor-2?concentration?and early childhood caries.; Membrane and soluble Toll‐like receptor 2 in patients with psoriasis treated by Goeckerman therapy; The discriminative capacity of soluble Toll-like receptor (sTLR)2 and sTLR4 in inflammatory diseases; Soluble Toll-Like Receptors 2 and 4 in Cerebrospinal Fluid of Patients with Acute Hydrocephalus followingAneurysmal Subarachnoid Haemorrhage.; The Effect of Rosuvastatin on Markers of Immune Activation in Treatment-Naive Human Immunodeficiency Virus-Patients; Pentraxin 3 and other inflammatory biomarkers related to atrial fibrillation in cardiac surgery; Soluble TLR2 and 4 concentrations in cerebrospinal fluid in HIV/SIV-relatedneuropathological conditions.; Toll-like receptors and their soluble forms differ in the knee and thumb basal osteoarthritic joints; Features of the Pattern Recognition Receptor TLR-2 Expression and Cytokines IL-2, IL-4 Profile in Children with Food Hypersensitivity; Prospective study on the significance of mucosal barrier dysfunction markers in assessing the effectiveness of the new strategy for treating food hypersensitivity in …; Vinpocetine regulates levels of circulating TLRs in Parkinson's disease patients; PROGNOSTIC ROLE OF IL-4, IL-2 AND TLR-2 FOR PREDICTING OF COURSE OF FOOD ALLERGY IN CHILDREN; STUDY OF THE ACTION OF CHROMIUM-CONTAINING BIOPREPARATIONS FROM SPIRULINA ON INSULIN ACTIVITY IN RATS;