Item no. |
SEA160Ra-48T |
Manufacturer |
Cloud-Clone
|
Amount |
48T |
Category |
|
Type |
Elisa |
Specific against |
Rat |
Sensitivity |
The minimum detectable dose of this kit is typically less than 6.0pg/mL |
ECLASS 10.1 |
32160605 |
ECLASS 11.0 |
32160605 |
UNSPSC |
41116126 |
Alias |
SERPINF2; AAP; A2-PI; API; PLI; A2AP; Alpha-2-Plasmin Inhibitor; Serpin Peptidase Inhibitor Clade F Member 2; Pigment Epithelium Derived Factor |
Available |
|
Specificity |
This assay has high sensitivity and excellent specificity for detection of Alpha 2-Antiplasmin (a2PI). No significant cross-reactivity or interference between Alpha 2-Antiplasmin (a2PI) and analogues was observed. |
Manufacturers Category |
ELISA Kit |
Application |
Enzyme-linked immunosorbent assay for Antigen Detection. |
Organism species |
Rattus norvegicus (Rat) |
Sample type |
serum, plasma, tissue homogenatesand other biological fluids |
Assay length |
3h |
Method |
Double-antibody Sandwich |
Precision |
Intra-assay: CV<10% Inter-Assay: CV<12% |
Stability |
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end. |
Assay procedure summary |
1. Prepare all reagents, samples and standards; 2. Add 100uL standard or sample to each well. Incubate 2 hours at 37°C; 3. Aspirate and add 100uL prepared Detection Reagent A. Incubate 1 hour at 37°C; 4. Aspirate and wash 3 times; 5. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37°C; 6. Aspirate and wash 5 times; 7. Add 90uL Substrate Solution. Incubate 10-20 minutes at 37°C; 8. Add 50uL Stop Solution. Read at 450nm immediately. |
Test principle |
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Alpha 2-Antiplasmin (a2PI). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Alpha 2-Antiplasmin (a2PI). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Alpha 2-Antiplasmin (a2PI), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Alpha 2-Antiplasmin (a2PI) in the samples is then determined by comparing the O.D. of the samples to the standard curve. |
Item Name |
Alpha 2-Antiplasmin |
Manufacturers Research Field |
Hematology; |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.