ELISA Kit for Matrix Metalloproteinase 2 (MMP2)

0.312-20ng/mL

The minimum detectable dose of this kit is typically less than 0.134ng/mL

3h

This assay has high sensitivity and excellent specificity for detection of Matrix Metalloproteinase 2 (MMP2).
No significant cross-reactivity or interference between Matrix Metalloproteinase 2 (MMP2) and analogues was observed.

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Matrix Metalloproteinase 2 (MMP2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Matrix Metalloproteinase 2 (MMP2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Matrix Metalloproteinase 2 (MMP2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Matrix Metalloproteinase 2 (MMP2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Early outcome and blood-brain barrier integrity after co-administered thrombolysis and hyperbaric oxygenation in experimental stroke; The protective effect of dexmedetomidine on bupivacaine-induced sciatic nerve inflammation is mediated by mast cells; The unwounded skin remodeling in animal models of diabetes types 1 and 2.; Resveratrol reduces matrix metalloproteinases and alleviates intrahepatic cholestasis of pregnancy in rats; Evodiamine Attenuates PDGF-BB-Induced Migration of Rat Vascular Smooth Muscle Cells through Activating PPARγ; Research Article Effects of DNMT and HDAC Inhibitors (RG108 and Trichostatin A) on NGF-induced Neurite Outgrowth and Cellular Migration; Epigallocatechin Gallate Extends the Therapeutic Window of Recombinant Tissue Plasminogen Activator Treatment in Ischemic Rats; Anti-inflammatory and Anti-apoptotic Effect of Valproic Acid and Doxycycline Independent from MMP Inhibition in Early Radiation Damage; Pleiotropic effects of survivin in vascular endothelial cells.; Lysyl oxidase is involved in synovial hyperplasia and angiogenesis in rats with collagen‑induced arthritis; Liraglutide ameliorated peripheral neuropathy in diabetic rats: Involvement of oxidative stress, inflammation and extracellular matrix remodeling; Extracellular Matrix Remodeling and Modulation of Inflammation and Oxidative Stress by Sulforaphane in Experimental Diabetic Peripheral Neuropathy; Hepatoprotectivity of Panduratin A against liver damage: demonstration with a rat model of cirrhosis induced by thioacetamide;