ELISA Kit for Connective Tissue Growth Factor (CTGF)

7.8-500pg/mL

The minimum detectable dose of this kit is typically less than 3.5pg/mL

3h

This assay has high sensitivity and excellent specificity for detection of Connective Tissue Growth Factor (CTGF).
No significant cross-reactivity or interference between Connective Tissue Growth Factor (CTGF) and analogues was observed.

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Connective Tissue Growth Factor (CTGF). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Connective Tissue Growth Factor (CTGF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Connective Tissue Growth Factor (CTGF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Connective Tissue Growth Factor (CTGF) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Promotion of bone formation by naringin in a titanium particle-induced diabetic murine calvarial osteolysis model; Connective tissue growth factor hammerhead ribozyme attenuates human hepatic stellate cell function; Activation of JNK Signaling Mediates Connective Tissue Growth Factor Expression and Scar Formation in Corneal Wound Healing; Clinical significance of inflammatory and fibrogenic cytokines in diabetic nephropathy; Connective tissue growth factor (CTGF/CCN2): A protagonist in cardiac allograft vasculopathy development?; Concentrations of connective tissue growth factor in patients with nonalcoholic fatty liver disease: Association with liver fibrosis; Clinical significance of connective tissue growth factor in hepatitis B virus-induced hepatic fibrosis; Cytokine levels as biomarkers of radiation fibrosis in patients treated with breast radiotherapy; Connective tissue growth factor and cardiac diastolic dysfunction: human data from the Taiwan Diastolic Heart Failure Registry and molecular basis by cellular and animal models; Disruption of collagen homeostasis can reverse established age-related myocardial fibrosis; Fibrosis of extracellular matrix is related to the duration of the disease but is unrelated to the dynamics of collagen metabolism in dilated cardiomyopathy; Left ventricular reverse remodeling is not related to biopsy-detected extracellular matrix fibrosis and serum markers of fibrosis in dilated cardiomyopathy, regardless of the definition used for LVRR; The relationship between anti-vascular endothelial growth factor and fibrosis in proliferative retinopathy: clinical and laboratory evidence; Molecular mechanisms underlying fibrosis and elastin destruction in childhood interstitial lung diseases; Left ventricular reverse remodeling is not relatedto biopsy‑detected extracellular matrix fibrosis and serummarkers of fibrosis in dilated cardiomyopathy, regardlessof the definition used for LVRR; 12-month patterns of serum markers of collagen synthesis, transforming growth factor and connective tissue growth factor are similar in new-onset and chronic dilated cardiomyopathy in patients both with and without cardiac fibrosis.; Prognostic value of fibrosis-related markers in dilated cardiomyopathy: A link between osteopontin and cardiovascular events.; TGFβ mediates collagen production in human CRSsNP nasal mucosa‐derived fibroblasts through Smad2/3‐dependent pathway and CTGF induction and secretion; Combination of Plasma Biomarkers and Clinical Data for the Detection of Myocardial Fibrosis or Aggravation of Heart Failure Symptoms in Heart Failure with …;