Item no. |
SCB388Mu-10x96T |
Manufacturer |
Cloud-Clone
|
Amount |
10x96T |
Category |
|
Type |
Elisa-Kit |
Specific against |
Mouse |
ECLASS 10.1 |
32160605 |
ECLASS 11.0 |
32160605 |
UNSPSC |
41116126 |
Alias |
Lipocalin-2, LCN2, p25, Lipocalin 2, Oncogene 24p3, 25 kDa alpha-2-microglobulin-related subunit of MMP-9, Siderocalin LCN2 |
Available |
|
Quantity options |
|
Detection range |
27.4-20, 000pg/mL |
Organism species |
Mus musculus (Mouse) |
Sensitivity |
The minimum detectable dose of this kit is typically less than 10.2pg/mL |
Sample type |
serum, plasma, urine, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
Assay length |
2h, 40min |
Method |
Double-antibody Sandwich |
Specificity |
This assay has high sensitivity and excellent specificity for detection of Neutrophil gelatinase-associated lipocalin (NGAL). No significant cross-reactivity or interference between Neutrophil gelatinase-associated lipocalin (NGAL) and analogues was observed. |
Precision |
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Neutrophil gelatinase-associated lipocalin (NGAL) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Neutrophil gelatinase-associated lipocalin (NGAL) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12% |
Stability |
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end. |
Assay procedure summary |
1. Prepare all reagents, samples and standards; 2. Add 100µ L standard or sample to each well. Incubate 2 hours at 37° C; 3. Aspirate and add 100µ L prepared Detection Reagent A. Incubate 1 hour at 37° C; 4. Aspirate and wash 3 times; 5. Add 100µ L prepared Detection Reagent B. Incubate 30 minutes at 37° C; 6. Aspirate and wash 5 times; 7. Add 100µ L Substrate Solution. Incubate 10 minutes at 37° C; 8. Read RLU value immediately. |
Test principle |
The microplate provided in this kit has been pre-coated with an antibody specific to Neutrophil gelatinase-associated lipocalin (NGAL). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Neutrophil gelatinase-associated lipocalin (NGAL). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Neutrophil gelatinase-associated lipocalin (NGAL) level in the sample or standard.; |
Research Area |
Infection immunity; |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.