« Back
Home /
CLIA Kit for Glycated Hemoglobin A1c (HbA1c)

CLIA Kit for Glycated Hemoglobin A1c (HbA1c)

Item no.	SCA190Hu-5x96T
Manufacturer	Cloud-Clone
Amount	5x96T
Category	ELISA & Immunoassays Immunoassay CLIA Kits CLIA
Type	Clia
Applications	CLIA
Specific against	Human
ECLASS 10.1	32161000
ECLASS 11.0	32161000
UNSPSC	41116126
Alias	Glycosylated Hemoglobin, Hemoglobin A1c, Hb1c, HbAIc, HbAIc
Available
Quantity options
Quantity options
Alternative Names	Glycosylated Hemoglobin, Hemoglobin A1c, Hb1c, HbAIc, HbAIc
Detection range	0.82-600ng/mL The standard curve concentrations used for the CLIA’s were 600ng/mL, 200ng/mL, 66.67ng/mL, 22.22ng/mL, 7.41ng/mL, 2.47ng/mL, 0.82ng/mL
Sensitivity	The minimum detectable dose of this kit is typically less than 035ng/mL
Sample type	Plasma
Assay length	4.5 hours
Method	Sandwich
Specificity	This assay has high sensitivity and excellent specificity for detection of Glycated Hemoglobin A1c (HbA1c). No significant cross-reactivity or interference between Glycated Hemoglobin A1c (HbA1c) and analogues was observed.
Precision	Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Glycated Hemoglobin A1c (HbA1c) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Glycated Hemoglobin A1c (HbA1c) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Stability	The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Assay procedure summary	1. Prepare all reagents, samples and standards; 2. Add 100µ L standard or sample to each well. Incubate 2 hours at 37^oC; 3. Aspirate and add 100µ L prepared Detection Reagent A. Incubate 1 hour at 37^oC; 4. Aspirate and wash 3 times; 5. Add 100µ L prepared Detection Reagent B. Incubate 30 minutes at 37^oC; 6. Aspirate and wash 5 times; 7. Add 100µ L Substrate Solution. Incubate 10 minutes at 37^oC; 8. Read RLU value immediately.
Test principle	The microtiter plate provided in this kit has been pre-coated with an antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated secondary antibody. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Fibrinogen (FG) level in the sample or standard.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Request Data Sheet

Request product datasheet

Request safety datasheet

Simple Order Order Subscription

Amount: 5x96T

available

Delivery expected until 10/4/2024

Express Delivery: Save 3 days

Compare

Add to wishlist

Recommend by mail