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CLIA Kit for Collagen Type IV (COL4)

CLIA Kit for Collagen Type IV (COL4)

Item no.	SCA180Hu-5x96T
Manufacturer	Cloud-Clone
Amount	5x96T
Category	Cell Biology ELISA Cell Stainings ECM ELISA & Immunoassays ELISA Kits Immunoassay
Type	Elisa-Kit
Specific against	Human
ECLASS 5.1	32160605
ECLASS 6.1	32160605
ECLASS 8.0	32160605
ECLASS 9.0	32160605
ECLASS 10.0.1	32160605
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	Type-IV Collagen
Available
Quantity options
Detection range	0.14-100ng/mL
Organism species	Homo sapiens (Human)
Sensitivity	The minimum detectable dose of this kit is typically less than 0.07ng/mL
Sample type	Serum, plasma, tissue homogenates and other biological fluids
Assay length	2h, 40min
Method	Double-antibody Sandwich
Specificity	This assay has high sensitivity and excellent specificity for detection of Collagen Type IV (COL4). No significant cross-reactivity or interference between Collagen Type IV (COL4) and analogues was observed.
Precision	Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Collagen Type IV (COL4) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Collagen Type IV (COL4) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Stability	The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Assay procedure summary	1. Prepare all reagents, samples and standards; 2. Add 100µ L standard or sample to each well. Incubate 2 hours at 37° C; 3. Aspirate and add 100µ L prepared Detection Reagent A. Incubate 1 hour at 37° C; 4. Aspirate and wash 3 times; 5. Add 100µ L prepared Detection Reagent B. Incubate 30 minutes at 37° C; 6. Aspirate and wash 5 times; 7. Add 100µ L Substrate Solution. Incubate 10 minutes at 37° C; 8. Read RLU value immediately.
Test principle	The microplate provided in this kit has been pre-coated with an antibody specific to Collagen Type IV (COL4). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Collagen Type IV (COL4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Collagen Type IV (COL4) level in the sample or standard.;
Research Area	Tumor immunity; Hepatology;
Reference	Key Matrix Proteins Within the Pancreatic Islet Basement Membrane Are Differentially Digested During Human Islet Isolation.;

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

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