Item no.
MEA226Ra-24T
Manufacturer
Cloud-Clone
Amount
24T
Category
Type
Elisa-Kit
Specific against
Rat
ECLASS 10.1
32160605
ECLASS 11.0
32160605
UNSPSC
41116126
Alias
FFA3R; GPR41; G Protein-Coupled Receptor 41
Available
Quantity options
Detection range
0.156-10ng/mL
Organism species
Rattus norvegicus (Rat)
Sensitivity
The minimum detectable dose of this kit is typically less than 0.059ng/mL
Sample type
Tissue homogenates, cell lysates and other biological fluids
Assay length
3h
Method
Double-antibody Sandwich
Specificity
This assay has high sensitivity and excellent specificity for detection of Mini Samples Free Fatty Acid Receptor 3 (FFAR3). No significant cross-reactivity or interference between Mini Samples Free Fatty Acid Receptor 3 (FFAR3) and analogues was observed.
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mini Samples Free Fatty Acid Receptor 3 (FFAR3) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mini Samples Free Fatty Acid Receptor 3 (FFAR3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Assay procedure summary
1. Prepare all reagents, samples and standards; 2. Add 25µ L standard or sample to each well. Incubate 1 hour at 37° C; 3. Aspirate and add 25µ L prepared Detection Reagent A. Incubate 1 hour at 37° C; 4. Aspirate and wash 3 times; 5. Add 25µ L prepared Detection Reagent B. Incubate 30 minutes at 37° C; 6. Aspirate and wash 5 times; 7. Add 25µ L Substrate Solution. Incubate 10-20 minutes at 37° C; 8. Add 20µ L Stop Solution. Read at 450nm immediately.
Test principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mini Samples Free Fatty Acid Receptor 3 (FFAR3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mini Samples Free Fatty Acid Receptor 3 (FFAR3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mini Samples Free Fatty Acid Receptor 3 (FFAR3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mini Samples Free Fatty Acid Receptor 3 (FFAR3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Research Area
CD & Adhesion molecule;
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.