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ELISA Kit DIY Materials for Heat Shock Protein 27 (Hsp27)

ELISA Kit DIY Materials for Heat Shock Protein 27 (Hsp27)

Manufacturer	Cloud-Clone
Category	ELISA & Immunoassays ELISA Kits
Type	Elisa-Kit
Specific against	Mouse
Amount	20 plates
Item no.	KSA693Mu01-20plates
eClass 6.1	32160605
eClass 9.0	32160605
Available
Alias	HSPB1; HSP-B1; CMT2F; HSP28; Hsp25; Heat Shock 27kDa Protein 1; 28 kDa heat shock protein; Heat shock protein beta-1; Estrogen-regulated 24 kDa protein;
Item Name	Heat Shock Protein 27
Reagent Contents	Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
Usage	1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours. 2. Aspirate and wash 1 time. 3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours. 4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
Specificity	The Abs in the kit have high sensitivity and excellent specificity for detection of Heat Shock 27kDa Protein 1 (HSPB1). No significant cross-reactivity or interference between Heat Shock 27kDa Protein 1 (HSPB1) and analogues was observed.
Detectable Sample	Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Applications	Main materials for "Do It(ELISA Kit) Yourself"
Applicable Principle	Double-antibody Sandwich ELISA for Antigen Detection
Detectable Range	0.78-50ng/mL
Applicable Sensitivity	0.32ng/mL
Research Area	Signal transduction; Tumor immunity; Developmental science;

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 20 plates

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Delivery expected until 8/2/2024

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