KRIBIOLISA™ Protein A ELISA (natural & structurally conserved recombinant Protein A specific)

Krishgen-Biopharma-Others; BIOPHARMA ASSAYS ; Drug Residue Assays / Bioprocess Contamination Assays ; Bioprocess Contamination Assays

Immunoassay for the measurement Naturaltructurally conserved Recombinant Protein A

Introduction:
A variety of antibodies are used as therapeutics agent as well as in pharmaceuticals. These antibodies are
generally purified through protein A column chromatography. This method of purification has the potential with
contamination of protein A, which might get leached out of the column and co-eluted with purified antibody. This
may result in adverse toxic or immunological reactions that ultimately affect the efficacy of the product antibody.
The highly sensitive Protein A ELISA is a quantitative, simple and will aid in monitoring the purification process as
well as screening product prior to its use.

Intended Use:
This kit is used for determination of natural and recombinant constructs of Protein A ligand contamination, in
various antibody products that are purified through Protein A chromatographic techniques.

Principle:
This assay is based on the principle of Sandwich ELISA. Samples containing Protein A are reacted with antiProtein A:HRP antibody simultaneously in the microtiter wells already coated with affinity purified capture antiProtein A antibody. This immunological reaction results in formation of a sandwich complex of solid phase
antibody-Protein-enzyme labeled antibody. The wells are washed to remove any unbound reactants as per the
wash procedure (see Assay procedure section mentioned below). TMB substrate is then added. Color develops
proportionally to the amount of Protein A in the samples. Color development is stopped by addition of stop
solution. Absorbance is measured at 450 nm