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CLIA Kit for Spermidine (SMD)

CLIA Kit for Spermidine (SMD)

Item no.	CCX053Ge-24T
Manufacturer	Cloud-Clone
Amount	24 T
Quantity options	10x96 T 24 T 48 T 5x96 T 96 T
Category	ELISA & Immunoassays Immunoassay CLIA Kits CLIA
Type	Clia
Applications	IA
Specific against	other
Sensitivity	The minimum detectable dose of this kit is typically less than 284.75pg/mL
ECLASS 10.1	32161000
ECLASS 11.0	32161000
UNSPSC	41116126
Available
Specificity	Pan-species (General)
Quantity options
Detection range	781.25-200, 000pg/mL
Organism species	Pan-species (General)
Sensitivity	The minimum detectable dose of this kit is typically less than 284.75pg/mL
Sample type	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length	2h
Method	Competitive Inhibition
Specificity	This assay has high sensitivity and excellent specificity for detection of Spermidine (SMD). No significant cross-reactivity or interference between Spermidine (SMD) and analogues was observed.
Precision	Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Spermidine (SMD) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Spermidine (SMD) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Stability	The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Assay procedure summary	1. Prepare all reagents, samples and standards; 2. Add 50µ L standard or sample to each well. And then add 50µ L prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37° C; 3. Aspirate and wash 3 times; 4. Add 100µ L prepared Detection Reagent B. Incubate 30 minutes at 37° C; 5. Aspirate and wash 5 times; 6. Add 100µ L Substrate Solution. Incubate 10 minutes at 37° C; 7. Read RLU value immediately.
Test principle	The microplate provided in this kit has been pre-coated with a monoclonal antibody specific to Spermidine (SMD). A competitive inhibition reaction is launched between biotin labeled Spermidine (SMD) and unlabeled Spermidine (SMD) (Standards or samples) with the pre-coated antibody specific to Spermidine (SMD). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Spermidine (SMD) in the sample. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is reverse proportional to the Spermidine (SMD) level in the sample or standard.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.