Item no. |
CCA393Ra-10x96T |
Manufacturer |
Cloud-Clone
|
Amount |
10x96 T |
Quantity options |
10x96 T
24 T
48 T
5x96 T
96 T
|
Category |
|
Type |
Elisa-Kit |
Applications |
IA |
Specific against |
Rat (Rattus norvegicus) |
Sensitivity |
The minimum detectable dose of this kit is typically less than 1.5pg/mL |
ECLASS 10.1 |
32160605 |
ECLASS 11.0 |
32160605 |
UNSPSC |
41116126 |
Available |
|
Specificity |
Rattus norvegicus (Rat) |
Quantity options |
|
Detection range |
3.9-1, 000pg/mL |
Organism species |
Rattus norvegicus (Rat) |
Sensitivity |
The minimum detectable dose of this kit is typically less than 1.5pg/mL |
Sample type |
serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
Assay length |
2h |
Method |
Competitive Inhibition |
Specificity |
This assay has high sensitivity and excellent specificity for detection of Substance P (SP). No significant cross-reactivity or interference between Substance P (SP) and analogues was observed. |
Precision |
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Substance P (SP) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Substance P (SP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12% |
Stability |
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end. |
Assay procedure summary |
1. Prepare all reagents, samples and standards; 2. Add 50µ L standard or sample to each well. And then add 50µ L prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37° C; 3. Aspirate and wash 3 times; 4. Add 100µ L prepared Detection Reagent B. Incubate 30 minutes at 37° C; 5. Aspirate and wash 5 times; 6. Add 100µ L Substrate Solution. Incubate 10 minutes at 37° C; 7. Read RLU value immediately. |
Test principle |
The microplate provided in this kit has been pre-coated with a monoclonal antibody specific to Substance P (SP). A competitive inhibition reaction is launched between biotin labeled Substance P (SP) and unlabeled Substance P (SP) (Standards or samples) with the pre-coated antibody specific to Substance P (SP). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Substance P (SP) in the sample. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is reverse proportional to the Substance P (SP) level in the sample or standard. |
Research Area |
Endocrinology; Neuro science; Hormone metabolism; |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.