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COVID-19 Human IgG ELISA Kit

COVID-19 Human IgG ELISA Kit

Item no.	IEQ-CoVS1RBD-IgG-1
Manufacturer	Raybiotech
Amount	1 Plate Kit
Category	Infectious Diseases Viral Infections Coronavirus (COVID-19) ELISA ELISA & Immunoassays ELISA Kits Immunoassay
Type	Elisa-Kit
Specific against	Human (Homo sapiens)
Host	Hamster - Armenian
Dry ice	Yes
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Shipping condition	Dry ice
Available
Compatible Sample Types	Dried Blood Plasma Saliva Serum
Design Principle	Indirect
Method of Detection	Colorimetric
Quantitative/Semi-Quantitative	Quantitative
Solid Support	96-well Microplate
Gene Symbols	IGG
Kit Components	S A R S- Co V-2 N-protein coated 96-well microplate Wash Buffer Positive Control Biotinylated Anti- Human Ig G H R P- Streptavidin concentrate T M B One- Step Substrate Stop Solution Assay Diluent5 X Sample Diluent1 X P B S ( D B S version) Tween-20 ( D B S version) Protease Inhibitor Cocktail ( D B S version)
Other Materials Required	Microplate reader capable of measuring absorbance at 450 nm Precision pipettes to deliver 2 Âµl to 1 ml volumes Adjustable 1-25 ml pipettes for reagent preparation100 ml and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Distilled or deionized water
Protocol Outline	Prepare all reagents, samples and standards as instructed. Add 100 Âµl positive control or sample to each well. Incubate 1 hour at room temperature. Add 100 Î¼l prepared Biotinylated Anti- Human Ig G Antibody into each well. Incubate 30 minutes at room temperature. Add 100 Î¼l prepared H R P- Streptavidin solution to each well. Incubate 30 minutes at room temperature. Add 100 Âµl T M B One- Step Substrate Reagent to each well. Incubate 15 minutes at room temperature. Add 50 Âµl Stop Solution to each well. Read at 450 nm immediately.
Storage/Stability	The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. For extended storage, it is recommended to store at -80°C.
Note	This Product is for Research Use Only, not for use in in vitro diagnostics, following guidance from the FDA for tests submitted to the FDA for Emergency Use Authorization and Notification on March 16th, 2020, and amended on May 4th, 2020. While this test has been submitted for FDA EUA Authorization, it currently has not been reviewed by the FDA and results from antibody testing should not be used as the sole basis to diagnose or exclude SARS-CoV-2 infection or to inform infection status. Please consult the manual for specific details and limitations of this test.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.