High Sensitive ELISA Kit for Melatonin (MT)

Enzyme-linked immunosorbent assay for Antigen Detection.

serum, plasma and other biological fluids

Competitive Inhibition

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to High Sensitive Melatonin (MT) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled High Sensitive Melatonin (MT) and unlabeled High Sensitive Melatonin (MT) (Standards or samples) with the pre-coated antibody specific to High Sensitive Melatonin (MT). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of High Sensitive Melatonin (MT) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of High Sensitive Melatonin (MT) in the sample.

Metabolic pathway; Tumor immunity; Neuro science; Hormone metabolism;