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Prostaglandin E2 ELISA Kit

Prostaglandin E2 ELISA Kit

Item no.	EIA-PGE2-5
Manufacturer	Raybiotech
Amount	5 Plate Kit
Quantity options	1 Plate Kit 2 Plate Kit 5 Plate Kit
Category	ELISA & Immunoassays Immunoassay EIA Kits
Type	EIA
Specific against	Human, Mouse, Rat
Sensitivity	12.3 pg/ml
ECLASS 10.1	32161000
ECLASS 11.0	32161000
UNSPSC	41116126
Shipping condition	Cool pack
Available
Specificity	This EIA kit is designed to detect human, mouse, and rat PGE2.
Species Detected	Human Mouse Rat
Compatible Sample Types	Cell Culture Supernatants Plasma Serum
Design Principle	Competition-based
Method of Detection	Colorimetric
Quantitative/Semi-Quantitative	Quantitative
Solid Support	96-well Microplate
Gene Symbols	PGE2
Kit Components	Pre- Coated 96-well Strip Microplate Wash Buffer Standard Peptide Assay Diluent(s) Biotinylated Peptide H R P- Streptavidin T M B One- Step Substrate Stop Solution Assay Diagram Positive Control Sample Capture Antibody User Manual
Other Materials Required	Distilled or deionized water Precision pipettes to deliver 2 µl to 1 ml volumes Adjustable 1-25 ml pipettes for reagent preparation100 ml and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Orbital shaker Aluminum foil Saran Wrap Absorbent paper Microplate reader capable of measuring absorbance at 450nm Sigma Plot software (or other software that can perform four-parameter logistic regression models)
Protocol Outline	Prepare all reagents, samples and standards as instructed. Add 100 µl detection antibody to each well. Incubate 1.5 h at R T or O/ N at 4° C. Add 100 µl standard or sample to each well. Incubate 2.5 h at R T. Add 100 µl prepared streptavidin solution. Incubate 45 min at R T. Add 100 µl T M B One- Step Substrate Reagent to each well. Incubate 30 min at R T. Add 50 µl Stop Solution to each well. Read plate at 450 nm immediately.
Product Features	Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Protein Name & Synonyms	Prostaglandin E2, PGE2, dinoprostone, (5Z, 11α, 13E, 15S)-11, 15-Dihydroxy-9-oxo-prosta-5, 13-dien-1-oic acid, Prostin E2, Cervidil, Propess
Recommended Dilution (serum & plasma)	Human: 4X / Mouse: 2X / Rat: 2X

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.