Comparison

ELISA Kit for Somatostatin (SST)

Item no. CEA592Mu-10x96T
Manufacturer Cloud-Clone
Amount 10x96T
Category
Type Elisa-Kit
Specific against Mouse
ECLASS 10.1 32160605
ECLASS 11.0 32160605
UNSPSC 41116126
Alias GHIH, SRIF, SMST, GHRIH, GHRIF, Growth Hormone Releasing Inhibiting Hormone, Somatotropin Release-Inhibiting Factor, Somatostatin-14, Somatostatin-28
Available
Quantity options
Detection range
6.17-500pg/mL
Organism species
Mus musculus (Mouse)
Sensitivity
The minimum detectable dose of this kit is typically less than 2.77pg/mL
Sample type
serum, plasma and other biological fluids
Assay length
2h
Method
Competitive Inhibition
Specificity

This assay has high sensitivity and excellent specificity for detection of Somatostatin (SST).
No significant cross-reactivity or interference between Somatostatin (SST) and analogues was observed.

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Somatostatin (SST) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Somatostatin (SST) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 50µ L standard or sample to each well.
        And then add 50µ L prepared Detection Reagent A immediately.
        Shake and mix. Incubate 1 hour at 37° C;
3. Aspirate and wash 3 times;
4. Add 100µ L prepared Detection Reagent B. Incubate 30 minutes at 37° C;
5. Aspirate and wash 5 times;
6. Add 90µ L Substrate Solution. Incubate 10-20 minutes at 37° C;
7. Add 50µ L Stop Solution. Read at 450 nm immediately.
Test principle
This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Somatostatin (SST) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Somatostatin (SST) and unlabeled Somatostatin (SST) (Standards or samples) with the pre-coated antibody specific to Somatostatin (SST). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Somatostatin (SST) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Somatostatin (SST) in the sample.
Research Area
Tumor immunity; Endocrinology; Hormone metabolism;
Reference

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 10x96T
Available: In stock
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