ELISA Kit for Procollagen III N-Terminal Propeptide (PIIINP)

Rattus norvegicus (Rat)

2h

This assay has high sensitivity and excellent specificity for detection of Procollagen III N-Terminal Propeptide (PIIINP).
No significant cross-reactivity or interference between Procollagen III N-Terminal Propeptide (PIIINP) and analogues was observed.

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Procollagen III N-Terminal Propeptide (PIIINP) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Procollagen III N-Terminal Propeptide (PIIINP) and unlabeled Procollagen III N-Terminal Propeptide (PIIINP) (Standards or samples) with the pre-coated antibody specific to Procollagen III N-Terminal Propeptide (PIIINP). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Procollagen III N-Terminal Propeptide (PIIINP) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Procollagen III N-Terminal Propeptide (PIIINP) in the sample.