Comparison

ELISA Kit for Luteinizing Hormone (LH)

Manufacturer Cloud-Clone
Category
Type Elisa-Kit
Specific against Mouse
Amount 5x96T
Item no. CEA441Mu-5x96T
eClass 6.1 32160605
eClass 9.0 32160605
Available
Alias ICSH, Lutropin, Interstitial Cell Stimulating Hormone
Quantity options
Detection range
370.4-30, 000pg/mL
Organism species
Mus musculus (Mouse)
Sensitivity
The minimum detectable dose of this kit is typically less than 145.2pg/mL
Sample type
serum, plasma and other biological fluids
Assay length
2h
Method
Competitive Inhibition
Specificity

This assay has high sensitivity and excellent specificity for detection of Luteinizing Hormone (LH).
No significant cross-reactivity or interference between Luteinizing Hormone (LH) and analogues was observed.

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Luteinizing Hormone (LH) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Luteinizing Hormone (LH) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 50µ L standard or sample to each well.
        And then add 50µ L prepared Detection Reagent A immediately.
        Shake and mix. Incubate 1 hour at 37° C;
3. Aspirate and wash 3 times;
4. Add 100µ L prepared Detection Reagent B. Incubate 30 minutes at 37° C;
5. Aspirate and wash 5 times;
6. Add 90µ L Substrate Solution. Incubate 10-20 minutes at 37° C;
7. Add 50µ L Stop Solution. Read at 450 nm immediately.
Test principle
This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Luteinizing Hormone (LH) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Luteinizing Hormone (LH) and unlabeled Luteinizing Hormone (LH) (Standards or samples) with the pre-coated antibody specific to Luteinizing Hormone (LH). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Luteinizing Hormone (LH) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Luteinizing Hormone (LH) in the sample.
Research Area
Endocrinology; Reproductive science; Hormone metabolism;
Reference
Effects of genistein and estrogen receptor subtype-specific agonists in ArKO mice following different administration routes.; PPARγ co-activator-1α co-activates steroidogenic factor 1 to stimulate the synthesis of luteinizing hormone and aldosterone; Effects of genistein and estrogen receptor subtype-specific agonists in ArKO mice following different administration routes; Inhibitory effects of mitotane on viability and secretory activity in mouse gonadotroph cell lines; Nanocomposites of bio-based hyperbranched polyurethane/funtionalized MWCNT as non-immunogenic, osteoconductive, biodegradable and biocompatible scaffolds in bone tissue engineering; Critical function of RA-GEF-2/Rapgef6, a guanine nucleotide exchange factor for Rap1, in mouse spermatogenesis; Targeted disruption of the mouse testis-enriched gene Znf230 does not affect spermatogenesis or fertility; Overexpression of PRL7D1 in Leydig Cells Causes Male Reproductive Dysfunction in Mice; A Jacob/Nsmf Gene Knockout Results in Hippocampal Dysplasia and Impaired BDNF Signaling in Dendritogenesis; Exposure of pregnant mice to perfluorobutanesulfonate causes hypothyroxinemia and developmental abnormalities in female offspring; New gonadotropin-releasing hormone glycolipids with direct antiproliferative activity and gonadotropin-releasing potency.; Pyrethroid Insecticide Cypermethrin Accelerates Pubertal Onset in Male Mice via Disrupting Hypothalamic-Pituitary-Gonadal Axis; Development of New Gonadotropin-Releasing Hormone-Modified Dendrimer Platforms with Direct Antiproliferative and Gonadotropin Releasing Activity; Pyrethroid Insecticide Cypermethrin Modulates Gonadotropin Synthesis via Calcium Homeostasis and ERK1/2 Signaling in LbT2 Mouse Pituitary Cells; Vitamin D deficiency impairs testicular development and spermatogenesis in mice; Lysophosphatidic acid acyltransferase 3 tunes the membrane status of germ cells by incorporating docosahexaenoic acid during spermatogenesis.; High sensitivity of testicular function to titanium nanoparticles; The Combination of icariin and constrained dynamic loading stimulation attenuates bone loss in ovariectomy‐induced osteoporotic mice; Transdermal Delivery of Luteinizing Hormone-releasing Hormone with Chitosan Microneedles: A Promising Tool for Androgen Deprivation Therapy; Effect of controlled ovarian hyperstimulation on puberty and estrus in mice offspring; Prenatal exposure to polychlorinated biphenyl and umbilical cord hormones and birth outcomes in an island population; Impact of Perfluorooctane Sulfonate on Reproductive Ability of Female Mice through Suppression of Estrogen Receptor a-Activated Kisspeptin Neurons; Impact of Triclosan on Female Reproduction through Reducing Thyroid Hormones to Suppress Hypothalamic Kisspeptin Neurons in Mice; A Pex13 knockout in germ cells induces a spermatogenic arrest; Crocetin attenuates DHT-induced polycystic ovary syndrome in mice via revising kisspeptin neurons; 基因敲除损伤小鼠睾丸睾酮产生与生精功能; Tanshinone IIA attenuates estradiol‑induced polycystic ovarian syndrome in mice by ameliorating FSHR expression in the ovary;

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 5x96T
Available: In stock
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