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Human Cardiac Troponin I (cTnI) ELISA Kit (CLIA)

Human Cardiac Troponin I (cTnI) ELISA Kit (CLIA)

Item no.	BM-EKU09460
Manufacturer	Biomatik
Amount	96T
Category	Cell Biology Cell Status Necrosis Cytoskeleton ELISA ELISA & Immunoassays ELISA Kits Immunoassay Microfilaments CLIA
Type	Elisa-Kit
Applications	CLIA
Specific against	Human
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Available
Format	96-Well Plate
Target Name	Cardiac Troponin I
Target Synonyms	TNNI3; TNNC1; CMH7; C-TnI; Troponin I Type 3; Troponin I Type 3, Cardiac
Sample type	Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Detection ra nge	2.74-2, 000 pg/mL
Sensitivity	0.92 pg/mL
Precision	Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cardiac Troponin I (cTnI) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cardiac Troponin I (cTnI) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Intra-assay: CV<10%. Inter-Assay: CV<12%.
Detection Method	Chemiluminescent
Assay Time	2 hours, 40min
Assay Type	Double-antibody Sandwich
Shipping Condition	Ice packs
Storage	Short term: 4CC; Long term: see manual.
Precaution of Use	The Stop Solution is acidic. Do not allow to contact skin or eyes.
Expiry Date	8 months
UniProt ID	P19429
Specificity	This assay has high sensitivity and excellent specificity for detection of Cardiac Troponin I (cTnI).
Cross Activity	This assay has high sensitivity and excellent specificity for detection of Cardiac Troponin I (cTnI).
Test principle	The microplate provided in this kit has been pre-coated with an antibody specific to Cardiac Troponin I (cTnI). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Cardiac Troponin I (cTnI). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Cardiac Troponin I (cTnI) level in the sample or standard.;
Assay procedure summary	1. Prepare all reagents, samples and standards; 2. Add 100 uL standard or sample to each well. Incubate 2 hours at 37CC; 3. Aspirate and add 100 uL prepared Detection Reagent A. Incubate 1 hour at 37CC; 4. Aspirate and wash 3 times; 5. Add 100 uL prepared Detection Reagent B. Incubate 30 minutes at 37CC; 6. Aspirate and wash 5 times; 7. Add 100 uL Substrate Solution. Incubate 10 minutes at 37CC; 8. Read RLU value immediately.
Stability	The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Restrictions	For Research Use Only. Not for use in diagnostic procedures, drug use, or for administration to humans or animals.
Quality Systems	The kit is manufactured at ISO 9001 and ISO 13485 certified facilities.
Quality Assurance	Biomatik ELISA Kits are not typically pre-assembled as finished products due to limited shelf life. Before shipping, each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Detection Range, Sensitivity, and Precision. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation. Please always refer to the hard copy manual included in The kitbefore experiment.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 96T

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