Rat TGF beta 1 ELISA Kit

This assay has shown no significant cross-reactivity.

Store at 2 - 8°C.

Rat TGF-β1 ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of rat TGF-β1 in the samples. A monoclonal antibody specific for rat TGF-β1 has been immobilized onto microwells. Standard orsamples are pipetted into the wells, and TGF-β1 present is bound by the immobilized antibody. After washing away any unbound substances, a biotin-linked monoclonal antibody specific for TGF-β1 is added to the wells. Following a wash to remove any unbound detect antibody, streptavidin-HRP is added. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of TGF-β1 bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.

Colorimetric

99% (Average Recovery from Serum Samples).