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EliKine™ Rat IL-4 ELISA Kit

EliKine™ Rat IL-4 ELISA Kit

Item no.	ABK-KTE9003-48T
Manufacturer	Abbkine Scientific
Amount	48T
Category	ELISA ELISA & Immunoassays ELISA Kits Immunoassay
Type	Elisa
Applications	ELISA
Specific against	Rat
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	IL 4; IL4; BCGF-1; BCGF1; BSF1; IL-4; MGC79402; B cell growth factor 1; B_cell stimulatory factor 1; lymphocyte stimulatory factor 1
Available
Applications Notes	EliKine™ Rat IL-4 ELISA Kit employs a two-site sandwich ELISA to quantitate IL-4 in samples. An antibody specific for IL-4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL-4 is added to the wells. After washing, proprietary EliKine™ Streptavidin-HRP conjugates is added to the wells. Following a wash to remove any unbound streptavidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL-4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection Method	Colorimetric
SampleType	Cell culture supernatants#Serum#Plasma#Other biological fluids
Assay Type	Sandwich ELISA (quantitative)
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit components	• Rat IL-4 microplate • Rat IL-4 standard • Rat IL-4 detect antibody • EliKine™ Streptavidin-HRP • Standard diluent • Assay buffer • HRP substrate • Stop solution • Wash buffer • Plate covers.
Features & Benefits	EliKine™ Rat IL-4 ELISA Kit has high sensitivity and excellent specificity for detection of Rat IL-4. No significant cross-reactivity or interference between Rat IL-4 and analogues was observed.
Calibration Range	1.56 pg/mL-100 pg/mL
Limit of detection	1.0 pg/mL
Usage Notes	• Do not mix components from different kit lots or use reagents beyond the kit expiration date. • Allow all reagents to warm to room temperature for at least 30 minutes before opening. • Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. • Unused wells must be kept desiccated at 4 °C in the sealed bag provided. • Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. • It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Interleukin 4 is a pleiotropic cytokine produced primarily by activated T lymphocytes, mast cells and basophils . IL-4 has multiple immune response-modulating functions on a variety of cell types. It is an important modulator of the differentiation of precursor T helper cells to the Th2 subset that mediates humoral immunity and modulates antibody production. In addition, IL-4 has also been shown to have anti-tumor activity both in vivoand in vitro.The biological effects of IL-4 are mediated by specific cell surface receptor complexes. One type of functional IL-4 receptor complex consists of the IL-4-binding subunit and a second chain, designated the common c chain because it has also been identified as a component of the receptor complexes for IL-4, IL-7, IL-9 and IL-15
Accession Link	http://www.uniprot.org/uniprot/P20096
Booklet	KTE9003-B.pdf
Tag	IL4
Description	EliKine™ Rat IL-4 ELISA Kit employs a two-site sandwich ELISA to quantitate Rat IL-4.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 48T

available

Delivery expected until 9/13/2024