EliKine™ Human Alpha Fetoprotein (AFP) ELISA Kit

EliKine™ Human Alpha Fetoprotein (AFP) ELISA Kit employs a double antibody sandwich method to quantitate Human AFP in samples. An antibody specific for Human AFP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Human AFP present is bound by the immobilized antibody. After removing any unbound substances, an HRP-conjugated antibody specific for Human AFP is added to the wells. After washing, remove any unbound HRP-conjugated antibody reagent, adding HRP Substrate (TMB), TMB turns blue under the catalysis of HRP, and turns yellow after adding stop solution. Measure the OD value with a microplate reader at 450 nm wavelength. The Human Alpha Fetoprotein (AFP) concentration is proportional to the OD450 nm value.

Serum#Plasma# Cell culture supernatants

Multiple steps standard sandwich ELISA assay with a working time of 3.5 hours. It depends on the experience of the operation person.

EliKine™ Human Alpha Fetoprotein (AFP) ELISA Kit has high sensitivity and excellent specificity for detection of Human Alpha Fetoprotein (AFP), No significant cross-reactivity or interference between Human Alpha Fetoprotein (AFP) and analogues was observed.

0.313 ng/mL

Stored at 4℃ for 12 months.

Alpha-fetoprotein (AFP) is a highly specific and sensitive tumor marker for primary liver cancer. Its Cutoff value is generally 20 μg/L, when more than 500 μg/L or content rising, more certain significance.

AFP