EliKine™ Human CRP ELISA Kit

EliKine™ Human CRP ELISA Kit employs a two-site sandwich ELISA to quantitate CRP in samples. An antibody specific for CRP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CRP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CRP is added to the wells. After washing, proprietary EliKine™ Streptavidin-HRP conjugates is added to the wells. Following a wash to remove any unbound streptavidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CRP bound in the initial step. The color development is stopped and the intensity of the color is measured.

Cell culture supernatants#Serum#Plasma#Other biological fluids

Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

EliKine™ Human CRP ELISA Kit has high sensitivity and excellent specificity for detection of Human CRP. No significant cross-reactivity or interference between Human CRP and analogues was observed.

8 pg/mL

The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

C-reactive protein encoded by CRP gene belongs to the pentaxin family. It is involved in several host defense related functions based on its ability to recognize foreign pathogens and damaged cells of the host and to initiate their elimination by interacting with humoral and cellular effector systems in the blood. Consequently, the level of C-reactive protein in plasma increases greatly during acute phase response to tissue injury, infection, or other inflammatory stimuli.

KTE6004-B.pdf

EliKine™ Human CRP ELISA Kit employs a two-site sandwich ELISA to quantitate Human CRP.