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Chicken Troponin C, skeletal muscle (TNNC2) ELISA Kit

Chicken Troponin C, skeletal muscle (TNNC2) ELISA Kit

Item no.	ABK-KTE30008-96Tx5
Manufacturer	Abbkine Scientific
Amount	96Tx5
Category	Cell Biology Cytoskeleton ELISA ELISA & Immunoassays ELISA Kits Immunoassay Microfilaments
Type	Elisa-Kit
Applications	ELISA
Specific against	Bovine
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	TNNC2, Fast skeletal muscle troponin C, troponin C2, fast
Available
Accession	P02588
Applications Notes	This Chicken Troponin C, skeletal muscle (TNNC2) ELISA Kit employs a two-site sandwich ELISA to quantitate TNNC2 in samples. An antibody specific for TNNC2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTNNC2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TNNC2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNNC2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich ELISA (quantitative)
Background	The troponin C protein is the calcium-binding subunit of the troponin complex. It occurs in 2 distinct isoforms: fast skeletal troponin C, which is expressed exclusively in fast-twitch skeletal muscle, and slow/cardiac troponin C, which is expressed in both cardiac and slow-twitch skeletal muscle. Gahlmann and Kedes (1990) described the structure and sequence of the gene for fast-twitch troponin C. By PCR-based analysis of a monochromosomal hybrid panel followed by analysis of the Genebridge 4 radiation hybrid panel, Townsend et al. (1997) mapped the TNNC2 gene to chromosome 20. Tiso et al. (1997) independently mapped the TNNC2 gene to 20q12-q13.11 using PCR of radiation hybrids.
Calibration Range	Please inquire
Description	This Chicken Troponin C, skeletal muscle (TNNC2) ELISA Kit employs a two-site sandwich ELISA to quantitate TNNC2.
Detection Method	Colorimetric
Features And Benefits	Chicken Troponin C, skeletal muscle (TNNC2) ELISA Kit has high sensitivity and excellent specificity for detection of Chicken TNNC2. No significant cross-reactivity or interference between Chicken TNNC2 and analogues was observed.
Gene ID	396434
Kit Components	- Chicken Troponin C, skeletal muscle microplate - Chicken Troponin C, skeletal muscle standard - Chicken Troponin C, skeletal muscle detect antibody - Streptavidin-HRP - Standard diluent - Assay buffer - HRP substrate - Stop solution - Wash buffer - Plate covers
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Sample Type	Cell culture supernatants#Serum#Plasma#Other biological fluids
Storage Instructions	The unopened kit should be stored at 2 - 8C. After opening, please store refer to protocols.
Tag	TNNC2
Usage Notes	- Do not mix components from different kit lots or use reagents beyond the kit expiration date. - Allow all reagents to warm to room temperature for at least 30 minutes before opening. - Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. - Unused wells must be kept desiccated at 4 C in the sealed bag provided. - Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. - It is recommended that all samples and standards be assayed in duplicate or triplicate.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 96Tx5

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Delivery expected until 10/18/2024

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