Item no. |
E-AB-30913-20 |
Manufacturer |
Elabscience
|
Amount |
20uL |
Category |
|
Type |
Antibody Polyclonal |
Applications |
WB, IF, ELISA, IHC-P |
Specific against |
Human, Mouse, Rat |
Host |
Rabbit |
Isotype |
IgG |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
C85740, Cell cycle checkpoint kinase, Checkpoint, S. pombe, homolog of, 1, Checkpoint kinase 1, Checkpoint kinase 1 homolog (S. pombe), CHEK 1, Chek1, Chk 1, Chk1, CHK1 checkpoint homolog (S. pombe), CHK1, EC 2.7.11.1, rad27, Serine/threonine protein kina |
Similar products |
Chk1, 1, CHK1, Cell cycle checkpoint kinase, EC 2.7.11.1, homolog of, S. pombe, S. pombe, Checkpoint, STT3, subunit of the oligosaccharyltransferase complex, Chek1, Checkpoint kinase 1, CHEK 1, Chk 1, rad27, Serine/threonine protein kinase Chk1, Serine/threonine-protein kinase CHK1, C85740, Checkpoint kinase 1 homolog (S. pombe), CHK1 checkpoint homolog (S. pombe), homolog A (S. cerevisiae) |
Available |
|
Research Areas |
Cancer, Epigenetics and Nuclear Signaling |
Synonyms |
C85740, Cell cycle checkpoint kinase, Checkpoint, S. pombe, homolog of, 1, Checkpoint kinase 1, Checkpoint kinase 1 homolog (S. pombe), CHEK 1, Chek1, Chk 1, Chk1, CHK1 checkpoint homolog (S. pombe), CHK1, EC 2.7.11.1, rad27, Serine/threonine protein kinase Chk1, Serine/threonine-protein kinase CHK1, STT3, subunit of the oligosaccharyltransferase complex, homolog A (S. cerevisiae) |
Swissprot |
O14757 |
Background |
Cell cycle events are regulated by the sequential activation and deactivation of cyclin dependent kinases (Cdks) and by proteolysis of cyclins. Chk1 and Chk2 are involved in these processes as regulators of Cdks. Chk1 and Chk2 both function as essential components in the G2 DNA damage checkpoint by phosphorylating Cdc25C in response to DNA damage. Phosphorylation inhibits Cdc25C activity, thereby blocking mitosis. Cdc25A, Cdc25B and Cdc25C protein tyrosine phosphatases function as mitotic activators by dephosphorylating Cdc2 p34 on regulatory tyrosine residues. It has also been shown that Chk1 can phosphorylate Wee 1 in vitro, providing evidence that the hyperphosphorylated form of Wee 1, seen in cells delayed by Chk1 overexpression, is due to phosphorylation by Chk1. |
Concentration |
1mg/mL |
Storage |
Store at -20C. Avoid freeze / thaw cycles. |
Immunogen |
Synthesized peptide derived from human Chk1 around the non-phosphorylation site of Ser317. |
Buffer |
PBS with 0.02% sodium azide, 0.5% BSA and 50% glycerol, pH7.4 |
Purification Method |
Affinity purification |
Dilution |
WB 1:500-1:2000, IHC 1:100-1:300, IF 1:200-1:1000, ELISA 1:10000 |
Conjugation |
Unconjugated |
Calculated MW |
54kDa |
Observed MW |
54kDa |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.