CHEK1 Polyclonal Antibody

C85740, Cell cycle checkpoint kinase, Checkpoint, S. pombe, homolog of, 1, Checkpoint kinase 1, Checkpoint kinase 1 homolog (S. pombe), CHEK 1, Chek1, Chk 1, Chk1, CHK1 checkpoint homolog (S. pombe), CHK1, EC 2.7.11.1, rad27, Serine/threonine protein kinase Chk1, Serine/threonine-protein kinase CHK1, STT3, subunit of the oligosaccharyltransferase complex, homolog A (S. cerevisiae)

Cell cycle events are regulated by the sequential activation and deactivation of cyclin dependent kinases (Cdks) and by proteolysis of cyclins. Chk1 and Chk2 are involved in these processes as regulators of Cdks. Chk1 and Chk2 both function as essential components in the G2 DNA damage checkpoint by phosphorylating Cdc25C in response to DNA damage. Phosphorylation inhibits Cdc25C activity, thereby blocking mitosis. Cdc25A, Cdc25B and Cdc25C protein tyrosine phosphatases function as mitotic activators by dephosphorylating Cdc2 p34 on regulatory tyrosine residues. It has also been shown that Chk1 can phosphorylate Wee 1 in vitro, providing evidence that the hyperphosphorylated form of Wee 1, seen in cells delayed by Chk1 overexpression, is due to phosphorylation by Chk1.

Store at -20C. Avoid freeze / thaw cycles.

PBS with 0.02% sodium azide, 0.5% BSA and 50% glycerol, pH7.4

WB 1:500-1:2000, IHC 1:100-1:300, IF 1:200-1:1000, ELISA 1:10000

54kDa