Item no. |
ELS-PKSM040831-200ug |
Manufacturer |
Elabscience
|
Amount |
200ug |
Category |
|
Type |
Proteins |
Specific against |
Mouse |
Host |
HEK293 cells |
ECLASS 10.1 |
32160409 |
ECLASS 11.0 |
32160409 |
UNSPSC |
12352202 |
Alias |
CD106,Vascular cell adhesion protein 1, Vcam1, L1CAM,VCMA1,Vcam-1 |
Similar products |
L1CAM, Vascular cell adhesion protein 1, Vcam1, CD106, Vcam-1, VCMA1 |
Available |
|
Synonym |
CD106, Vascular cell adhesion protein 1, Vcam1, L1CAM, VCMA1, Vcam-1 |
Storage |
according to manual |
Activity |
Measured by the ability of the immobilized protein to support adhesion of U937 human histiocytic lymphoma cells. When 5 x 10E4 cells/well are added to mouse VCAM1 coated plates (10 ug/ml with 100 ul/well), approximately 70%-80% cells will adhere after 1 hour at RT. |
Protein Construction |
A DNA sequence encoding the extracellular domain (Met 1-Glu 698) of mouse VCAM1 (NP_035823.3) was fused with the a polyhistidine tag at the C-terminus. |
Sequence |
Met 1-Glu 698 |
Fusion tag |
C-His |
Accession |
NP_035823.3 |
Shipping |
This product is provided as lyophilized powder which is shipped with ice packs. |
Purity |
> 97 % as determined by SDS-PAGE |
Endotoxin |
< 1.0 EU per ug of the protein as determined by the LAL method. |
Stability and Storage |
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80CC. Reconstituted protein solution can be stored at 4-8C for 2-7 days. Aliquots of reconstituted samples are stable at < -20C for 3 months. |
Mol Mass |
75.8 kDa |
AP Mol Mass |
90-100 kDa |
Formulation |
Lyophilized from sterile PBS, pH 7.4 |
Reconstitution |
Please refer to the printed manual for detailed information. |
Background |
Vascular cell adhesion molecule 1 (VCAM-1), also known as CD106, is a cell surface sialoglycoprotein belonging to the immunoglobulin superfamily. Two forms of VCAM-1 with either six or seven extracellular Ig-like domains are generated by alternative splicing, with the longer form predominant. VCAM-1 is an endothelial ligand for very late antigen-4 (VLA-4) and α 4ß 7 integrin expressed on leukocytes, and thus mediates leukocyte-endothelial cell adhesion and signal transduction. VCAM-1 expression is induced on endothelial cells during inflammatory bowel disease, atherosclerosis, allograft rejection, infection, and asthmatic responses. During these responses, VCAM-1 forms a scaffold for leukocyte migration. VCAM-1 also activates signals within endothelial cells resulting in the opening of an " endothelial cell gate" through which leukocytes migrate. VCAM-1 has been identified as a potential anti-inflammatory therapeutic target, the hypothesis being that reduced expression of VCAM-1 will slow the development of atherosclerosis. In addition, VCAM-1-activated signals in endothelial cells are regulated by cytokines indicating that it is important to consider both endothelial cell adhesion molecule expression and function during inflammatory processes.Immune Checkpoint Immunotherapy Cancer Immunotherapy Targeted Therapy |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.