Recombinant Mouse Smad5 Protein (His & GST Tag)

1110051M15Rik, AI451355, Dwf-C, Madh5, MusMLP

A DNA sequence encoding the mouse SMAD5 (P97454) (Thr2-Ser465) was expressed with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

N-His-GST

This product is provided as lyophilized powder which is shipped with ice packs.

< 1.0 EU per ug of the protein as determined by the LAL method.

79.9 kDa

Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 8.0, 10% glycerol

SMAD5 is a member of the SMAD family. Members of this family mediate signal transduction by the TGF-beta/activin/BMP-2/4 cytokine superfamily from receptor Ser/Thr protein kinases at the cell surface to the nucleus. SMAD5 is involved in the TGF-beta signaling pathway that results in an inhibition of the proliferation of hematopoietic progenitor cells. It is also involved in cell signalling and modulates signals of bone morphogenetic proteins (BMP's). The binding of ligands causes the oligomerization and phosphorylation of the SMAD5 protein. SMAD5 is a receptor regulated SMAD (R-SMAD) and is activated by bone morphogenetic protein type 1 receptor kinase.