Item no. |
ELS-PKSH033603-50ug |
Manufacturer |
Elabscience
|
Amount |
50ug |
Category |
|
Type |
Proteins |
Specific against |
Human |
Host |
Human Cells |
ECLASS 10.1 |
32160409 |
ECLASS 11.0 |
32160409 |
UNSPSC |
12352202 |
Alias |
Leukocyte immunoglobulin-like receptor subfamily B member 4, Mast cell surface glycoprotein Gp49B, CD85k, Lilrb4, Gp49b |
Similar products |
Leukocyte immunoglobulin-like receptor subfamily B member 4, Lilrb4, CD85k, Mast cell surface glycoprotein Gp49B, Gp49b |
Available |
|
Synonym |
Leukocyte immunoglobulin-like receptor subfamily B member 4, Mast cell surface glycoprotein Gp49B, CD85k, Lilrb4, Gp49b |
Storage |
according to manual |
Protein Construction |
Recombinant Human LILRB4 is produced by our Mammalian expression system and the target gene encoding Gln22-Glu259 is expressed with a 6His tag at the C-terminus. |
Sequence |
Gln22-Glu259 |
Fusion tag |
C-His |
Accession |
Q8NHJ6 |
Shipping |
This product is provided as lyophilized powder which is shipped with ice packs. |
Purity |
> 95% as determined by reducing SDS-PAGE. |
Endotoxin |
< 1.0 EU per ug as determined by the LAL method. |
Stability and Storage |
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80CC. Reconstituted protein solution can be stored at 4-8C for 2-7 days. Aliquots of reconstituted samples are stable at < -20C for 3 months. |
Mol Mass |
27.1 kDa |
AP Mol Mass |
37 kDa |
Formulation |
Lyophilized from a 0.2 um filtered solution of PBS; pH 7.4. |
Reconstitution |
Please refer to the printed manual for detailed information. |
Background |
Mouse Leukocyte Immunoglobulin-like Receptor Subfamily B Member 4 (LILRB4/CD85k/ILT3) is an approximately transmembrane glycoprotein that negatively regulates immune cell activation. Mouse LILRB4 consists of a 215 amino acid (aa) extracellular domain with two Ig-like domains; a 22 aa transmembrane segment; and a 75 aa cytoplasmic domain with 3 immunoreceptor tyrosine-based inhibitory motifs (ITIM). Within the ECD; mouse LILRB4 shares 45% and 77% aa sequence identity with human and rat LILRB4; respectively. Alternative splicing of mouse LILRB4 generates a potentially soluble isoform that lacks the transmembrane segment. LILRB4 is expressed on dendritic cells (DC); monocytes; macrophages; and vascular endothelial cells (EC). Ligation of LILRB4 triggers ITIM-mediated inhibition of cellactivating signaling; leading to enhanced immune tolerance and reduced allogeneic graft rejection. Soluble LILRB4 induces the differentiation of CD8+ T suppressor cells (Ts) that can inhibit the effector functions of CD4+ Th cells and CD8+ CTL. In turn; CD8+ Ts cells induce LILRB4 up-regulation and a tolerogenic phenotype in monocytes; DC; and EC. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.