Item no. |
ELS-PKSH032902-10ug |
Manufacturer |
Elabscience
|
Amount |
10ug |
Category |
|
Type |
Proteins |
Specific against |
Human |
Host |
Human Cells |
ECLASS 5.1 |
34160400 |
ECLASS 6.1 |
34160400 |
ECLASS 8.0 |
42020190 |
ECLASS 9.0 |
42020190 |
ECLASS 10.0.1 |
32160409 |
ECLASS 10.1 |
32160409 |
ECLASS 11.0 |
32160409 |
UNSPSC |
12352202 |
Alias |
Platelet endothelial cell adhesion molecule, PECAM-1, EndoCAM, GPIIA, PECA1, CD31, PECAM1 |
Similar products |
PECAM-1, PECAM1, CD31, EndoCAM, GPIIA, PECA1, Platelet endothelial cell adhesion molecule |
Available |
|
Synonym |
Platelet endothelial cell adhesion molecule, PECAM-1, EndoCAM, GPIIA, PECA1, CD31, PECAM1 |
Storage |
according to manual |
Protein Construction |
Recombinant Human Platelet Endothelial Cell Adhesion Molecule is produced by our Mammalian expression system and the target gene encoding Gln28-Lys601 is expressed with a 6His tag at the C-terminus. |
Sequence |
Gln28-Lys601 |
Fusion tag |
C-6His |
Accession |
P16284 |
Shipping |
This product is provided as lyophilized powder which is shipped with ice packs. |
Purity |
> 95 % as determined by reducing SDS-PAGE. |
Endotoxin |
< 1.0 EU per ug as determined by the LAL method. |
Stability and Storage |
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80CC. Reconstituted protein solution can be stored at 4-8C for 2-7 days. Aliquots of reconstituted samples are stable at < -20C for 3 months. |
Mol Mass |
65.3 kDa |
AP Mol Mass |
85-95 kDa |
Formulation |
Lyophilized from a 0.2 um filtered solution of PBS, pH7.4. |
Reconstitution |
Please refer to the printed manual for detailed information. |
Background |
Platelet Endothelial Cell Adhesion Molecule (PECAM1, CD31), is a heavily glycosylated transmembrane protein belonging to the immunoglobulin (Ig) superfamily of cell adhesion molecules. CD31 is composed of an extracellular domain (ECD) of 574 amino acids (aa) containing six Ig-like domains, a transmembrane domain, and a 118 aa cytoplasmic domain. CD31 is highly expressed on endothelial cells and at a lower level on platelets, granulocytes, macrophages, dendritic cells, T and B cells, and natural killer (NK) cells. It is involved in cell adhesion and is required for transepithelial migration of leukocytes (TEM). CD31 acts as a homophilic receptor through its extracellular domain and is involved in downstream signaling via its cytoplasmic domain. This domain contains highly conserved ITIM motifs which, once tyrosine phosphorylated, recruit and activate the signaling molecules Src and SHP2. The resulting inhibition of TCR signaling increases the activation threshold of T cells, thus reinforcing peripheral tolerance and preventing development of autoimmunity. CD31 additionally regulates immune responses by acting as a key inhibitory receptor in dendritic cell development. CD31 is required for the transendothelial migration of leukocytes through intercellular junctions of vascular endothelial cells. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.