Item no. |
ELS-PKSH031694-20ug |
Manufacturer |
Elabscience
|
Amount |
20ug |
Category |
|
Type |
Proteins |
Specific against |
Human |
Host |
HEK293 cells |
ECLASS 10.1 |
32160409 |
ECLASS 11.0 |
32160409 |
UNSPSC |
12352202 |
Alias |
Alpha-1-Antichymotrypsin, ACT, Cell Growth-Inhibiting Gene 24/25 Protein, Serpin A3, SERPINA3, AACT,GIG24,GIG25,MGC88254,SERPINA3 |
Similar products |
Alpha-1-Antichymotrypsin, SERPINA3, AACT, ACT, GIG24, GIG25, MGC88254, Serpin A3, Cell Growth-Inhibiting Gene 24/25 Protein |
Available |
|
Synonym |
Alpha-1-Antichymotrypsin, ACT, Cell Growth-Inhibiting Gene 24/25 Protein, Serpin A3, SERPINA3, AACT, GIG24, GIG25, MGC88254, SERPINA3 |
Storage |
according to manual |
Activity |
Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2 (Anaspec, Catalog# 27114). The IC50 value is < 5 nM. |
Protein Construction |
A DNA sequence encoding the human SERPINA3 (NP_001076.2) (Met 1-Ala 423) was expressed, with a C-terminal polyhistidine tag. |
Sequence |
Met 1-Ala 423 |
Fusion tag |
C-His |
Accession |
NP_001076.2 |
Shipping |
This product is provided as lyophilized powder which is shipped with ice packs. |
Purity |
> 97 % as determined by reducing SDS-PAGE. |
Endotoxin |
< 1.0 EU per ug as determined by the LAL method. |
Stability and Storage |
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80CC. Reconstituted protein solution can be stored at 4-8C for 2-7 days. Aliquots of reconstituted samples are stable at < -20C for 3 months. |
Mol Mass |
46.5 kDa |
AP Mol Mass |
45 kDa |
Formulation |
Lyophilized from sterile 25mM HEPES, 0.15M NaCl, pH 7.8 |
Reconstitution |
Please refer to the printed manual for detailed information. |
Background |
SerpinA3, also known as Alpha 1-antichymotrypsin (AACT), is a plasma alpha globulin glycoprotein, and is a member of serpin superfamily of the serine protease inhibitors consisting of at least 35 members. SerpinA3 has been demonstrated to inhibit the activity of certain serine proteases, such as cathepsin G found in neutrophils, and chymases present in mast cells, by inducing a major conformational rearrangement, and thus protects some tissues from damage caused by proteolytic enzymes. This enzyme is produced primarily in the liver, and is identified as an acute-phase inflammatory protein. SerpinA3 deficiency has been associated with liver disease, and mutations of this gene have been observed in patients with Parkinson disease and chronic obstructive pulmonary disease. In addition, ACT gene polymorphism has been implicated with Alzheimer´s disease (AD), cerebral amyloid angiopathy (CAA), as well as stroke, since SerpinA3 is a major constituent of the plaques in AD and an inhibitor of amyloid beta peptide degradation. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.