Item no. |
ELS-PKSH030994-20ug |
Manufacturer |
Elabscience
|
Amount |
20ug |
Category |
|
Type |
Proteins |
Specific against |
Human |
Host |
HEK293 cells |
ECLASS 10.1 |
32160409 |
ECLASS 11.0 |
32160409 |
UNSPSC |
12352202 |
Alias |
Semaphorin-4D, A8,BB18, GR3, CD100,C9orf164,CD100,coll-4,M-sema-G,SEMAJ |
Similar products |
GR3, BB18, A8, CD100, SEMAJ, C9orf164, M-sema-G, coll-4, Semaphorin-4D |
Available |
|
Synonym |
Semaphorin-4D, A8, BB18, GR3, CD100, C9orf164, CD100, coll-4, M-sema-G, SEMAJ |
Storage |
according to manual |
Activity |
Measured by its binding ability in a functional ELISA. Immobilized human SEMA4D-His at 10 ug/ml (100 ul/well) can bind mouse PTPRC-Fc with a linear range of 0.625-5 ug/ml. |
Protein Construction |
A DNA sequence encoding the human SEMA4D isoform 1 (Q92854-1) extracellular domain (Met 1-Arg 734) was expressed, with a polyhistidine tag at the C-terminus. |
Sequence |
Met 1-Arg 734 |
Fusion tag |
C-His |
Accession |
Q92854-1 |
Shipping |
This product is provided as lyophilized powder which is shipped with ice packs. |
Purity |
> 92 % as determined by reducing SDS-PAGE. |
Endotoxin |
< 1.0 EU per ug of the protein as determined by the LAL method. |
Stability and Storage |
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80CC. Reconstituted protein solution can be stored at 4-8C for 2-7 days. Aliquots of reconstituted samples are stable at < -20C for 3 months. |
Mol Mass |
80.7 kDa |
AP Mol Mass |
110 kDa |
Formulation |
Lyophilized from sterile PBS, pH 7.4 |
Reconstitution |
Please refer to the printed manual for detailed information. |
Background |
SIGLEC5 contains 2 Ig-like C2-type (immunoglobulin-like) domains and 1 Ig-like V-type (immunoglobulin-like) domain. It belongs to the immunoglobulin superfamily and SIGLEC (sialic acid binding Ig-like lectin) family. SIGLEC5 is expressed by monocytic/myeloid lineage cells. It is found at high levels in peripheral blood leukocytes, spleen, bone marrow and at lower levels in lymph node, lung, appendix, placenta, pancreas and thymus. It is also expressed by monocytes and neutrophils but absent from leukemic cell lines representing early stages of myelomonocytic differentiation. SIGLEC5 is a putative adhesion molecule that mediates sialic-acid dependent binding to cells. It binds equally to alpha-2, 3-linked and alpha-2, 6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.