Recombinant Human TLK2/PKU-ALPHA Protein (His&GST Tag)

HsHPK, PKU-ALPHA

A DNA sequence encoding the amino acids (Leu 397-Asn 772) of human TLK2 (Q86UE8-1) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

N-His & GST

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs.Upon receipt; store it immediately at<-20CC.

< 1.0 EU per ug as determined by the LAL method.

71.3 kDa

Supplied as sterile 20mM Tris; 500mM NaCl; 0.5mM PMSF; pH 8.0

Serine / threonine-protein kinase tousled-like 2; also known as PKU-alpha; Tousled-like kinase 2 and TLK2; is a nucleus protein which belongs to the  protein kinase superfamily and Ser/Thr protein kinase family. The tousled-like kinases are an evolutionarily conserved family of proteins implicated in DNA repair; DNA replication and mitosis in metazoans and plants. Their absence from the yeasts and other eukaryotic 'microbes' suggests a specific role for them in the development of multicellular organisms. Tousled-like kinase 2 / TLK2 is widely expressed. It is present in fetal placenta; liver; kidney; pancreas; heart and skeletal muscle. It is also found in adult cell lines. Tousled-like kinase 2 / TLK2 contains one  protein kinase domain. Tousled-like kinase 2 / TLK2 is rapidly and transiently inhibited by phosphorylation following the generation of DNA double-stranded breaks during S-phase. This is cell cycle checkpoint and ATM-pathway dependent and appears to regulate processes involved in chromatin assembly.