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H-Ras Protein; wild type 6xHis Tag

H-Ras Protein; wild type 6xHis Tag

Item no.	RS01-A
Manufacturer	Cytoskeleton
Amount	1 x 100 ug
Quantity options	1 x 100 ug 3 x 100 ug
Category	Peptides & Proteins Recombinant Proteins Cell Biology Cytoskeleton small GTPases Ras Subfamily Rho Subfamily
Type	Proteins
Specific against	other
Purity	Purity is determined by scanning densitometry of proteins on SDS-PAGE gels. His-H-Ras samples are >80% pure.
Citations	Z. Surviladze et al. 2010. Identification of a small GTPase inhibitor using a high-throughput flow cytometry bead-based multiplex assay. J. Biomol. Screen. 15, 10-20.
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Alias	Small G protein, H-Ras protein, Hras, H Ras, His-H-Ras, wild type H-Ras
Similar products	Hras, Small G protein, H-Ras protein, H Ras, His-H-Ras, wild type H-Ras
Shipping condition	Room temperature
Available
Shipping Temperature	AT
Storage Conditions	On Arrival: 4°C
Delivery Time	1-2 Weeks
Weight (grams)	8
Product Uses	Ras biochemistry Ras GTPase assays Ras nucleotide exchange assays Ras binding studies
Material	The human H-Ras protein has been produced in a bacterial expression system. The protein is supplied as a lyophilized powder.When it is reconstituted in distilled water to 1 mg/ml, the protein is in the following buffer: 2 mM Tris pH 7.6, 0.5 mM MgCl₂ 0.5% sucrose, 0.1% dextran. Protein concentration is determined by the Precision Red Advanced Protein Assay Reagent, Cat. # ADV02. The recombinant protein is approximately 28 kDa, consisting of the H-Ras protein plus a histidine tag in the amino-terminus. For other forms of Ras as well as many other purified small G-proteins, see our main small G-protein product page.
Biological Activity	The biological activity of His-H-Ras is determined from its ability to catalyze the exchange of GDP for GTP. EDTA is used to sequester magensium ions from the His-H-Ras protein, thereby stimulating nucleotide exchange activity. The RhoGEF exchange assay Biochem Kit (Cat. # BK100) is used to monitor the nucleotide exchange in His-H-Ras.
Figure 1 Legend	Figure 1. His-H-Ras protein purity determination. A 10 ug sample of RS01 (His-H-Ras molecular weight approx. 28 kDa) was separated by electrophoresis in a 12% SDS-PAGE system. The protein was stained with Coomassie Blue.
Figure 2 Legend	His-Ras exchange assay using BK100. His-H-Ras protein (1 uM) was mixed with exchange buffer and aliquoted to four wells of a 96-well half area plate. After 5 cycles of reading in a fluorimeter, EDTA was added to 40 mM in the test samples and Milli-Q water to the control samples. Reactions were monitored for 30 min.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

RS01-A.pdf

RS01-A-datasheet.pdf

RS01-A-safety-datasheet.pdf