Item no. |
RH01-C |
Manufacturer |
Cytoskeleton
|
Amount |
3 x 100 ug |
Quantity options |
1 x 100 ug
3 x 100 ug
1 x 1 mg
|
Category |
|
Type |
Proteins |
Specific against |
other |
Purity |
Purity is determined by scanning densitometry of proteins on SDS-PAGE gels. His-RhoA samples are >85% pure. |
Citations |
Lee, J. H., Katakai, T., Hara, T., Gonda, H., Sugai, M. and Shimizu, A. (2004). Roles of p-ERM and Rho-ROCK signaling in lymphocyte polarity and uropod formation. J. Cell Biol. 167, 327-337. Kulkarni, S., Goll, D. E. and Fox, J. E. (2002). Calpain cleaves RhoA generating a dominant-negative form that inhibits integrin-induced actin filament assembly and cell spreading. J. Biol. Chem. 277, 24435-24441. |
ECLASS 10.1 |
32160409 |
ECLASS 11.0 |
32160409 |
UNSPSC |
12352202 |
Alias |
Small G protein, Wild type RhoA, RhoA, RhoA protein, Rho A, Rho, His-RhoA |
Similar products |
RhoA, Rho, Rho A, Small G protein, RhoA protein, Wild type RhoA, His-RhoA |
Shipping condition |
Room temperature |
Available |
|
Shipping Temperature |
AT |
Storage Conditions |
On Arrival: 4°C |
Delivery Time |
1-2 Weeks |
Weight (grams) |
24 |
Product Uses |
RhoA biochemistry RhoA GTPase assays RhoA nucleotide exchange assays RhoA binding studies |
Material |
The human RhoA protein has been produced in a bacterial expression system. The protein is supplied as a lyophilized powder.When it is reconstituted in distilled water to 1 mg/ml, the protein is in the following buffer: 2 mM Tris pH 7.6, 0.5 mM MgCl 2 0.5% sucrose, 0.1% dextran. Protein concentration is determined by the Precision Red Advanced Protein Assay Reagent, Cat. # ADV02. The recombinant protein is approximately 27 kDa, consisting of the RhoA protein plus a histidine tag in the amino-terminus. For other forms of RhoA as well as many other purified small G-proteins, see our main small G-protein product page. |
Biological Activity |
The biological activity of His-RhoA is determined from its ability to catalyze the exchange of GDP for GTP. The human Dbs (DH/PH) protein (Cat. # GE01) is an exchange factor for RhoA and Cdc42, and is used with the RhoGEF exchange assay Biochem Kit (Cat. # BK100) to monitor the exchange ability of His-RhoA. Stringent quality control ensures that the exchange rate (Vmax) of RhoA is enhanced eight fold in the presence of an equimolar amount of hDbs. |