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RhoA His Protein: wild type

RhoA His Protein: wild type

Item no.	RH01-A
Manufacturer	Cytoskeleton
Amount	1 x 100 ug
Quantity options	1 x 100 ug 3 x 100 ug 1 x 1 mg
Category	Peptides & Proteins Recombinant Proteins Cell Biology Cytoskeleton small GTPases Rho Subfamily
Type	Proteins
Specific against	other
Purity	Purity is determined by scanning densitometry of proteins on SDS-PAGE gels. His-RhoA samples are >85% pure.
Citations	Lee, J. H., Katakai, T., Hara, T., Gonda, H., Sugai, M. and Shimizu, A. (2004). Roles of p-ERM and Rho-ROCK signaling in lymphocyte polarity and uropod formation. J. Cell Biol. 167, 327-337. Kulkarni, S., Goll, D. E. and Fox, J. E. (2002). Calpain cleaves RhoA generating a dominant-negative form that inhibits integrin-induced actin filament assembly and cell spreading. J. Biol. Chem. 277, 24435-24441.
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Alias	Small G protein, Wild type RhoA, RhoA, RhoA protein, Rho A, Rho, His-RhoA
Similar products	RhoA, Rho, Rho A, Small G protein, RhoA protein, Wild type RhoA, His-RhoA
Shipping condition	Room temperature
Available
Shipping Temperature	AT
Storage Conditions	On Arrival: 4°C
Delivery Time	1-2 Weeks
Weight (grams)	9
Product Uses	RhoA biochemistry RhoA GTPase assays RhoA nucleotide exchange assays RhoA binding studies
Material	The human RhoA protein has been produced in a bacterial expression system. The protein is supplied as a lyophilized powder.When it is reconstituted in distilled water to 1 mg/ml, the protein is in the following buffer: 2 mM Tris pH 7.6, 0.5 mM MgCl₂ 0.5% sucrose, 0.1% dextran. Protein concentration is determined by the Precision Red Advanced Protein Assay Reagent, Cat. # ADV02. The recombinant protein is approximately 27 kDa, consisting of the RhoA protein plus a histidine tag in the amino-terminus. For other forms of RhoA as well as many other purified small G-proteins, see our main small G-protein product page.
Biological Activity	The biological activity of His-RhoA is determined from its ability to catalyze the exchange of GDP for GTP. The human Dbs (DH/PH) protein (Cat. # GE01) is an exchange factor for RhoA and Cdc42, and is used with the RhoGEF exchange assay Biochem Kit (Cat. # BK100) to monitor the exchange ability of His-RhoA. Stringent quality control ensures that the exchange rate (Vmax) of RhoA is enhanced eight fold in the presence of an equimolar amount of hDbs.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

RH01-A.pdf

RH01-A-datasheet.pdf

RH01-A-safety-datasheet.pdf