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Raf protein ras binding domain on GST beads

Raf protein ras binding domain on GST beads

Item no.	RF02-B
Manufacturer	Cytoskeleton
Amount	6 x 2 mg
Quantity options	2 x 2 mg 6 x 2 mg
Category	Oncology Neuroscience Targets for Rodent Neurosciences Neural Signaling By Cell Type Blastoma Carcinoma Glioma Hematology Lymphoma Myeloma Melanoma Sarcoma Cell Biology Cytoskeleton Reagents & Chemicals Astrocytoma Nanoparticles Beads & Resin By Organ Hepatic Cancer small GTPases Rho Subfamily
Type	Proteins
Specific against	other
Purity	Protein purity is determined by scanning densitometry of Coomassie Blue stained protein on a 12% SDS polyacrylamide gel. GST-Raf-RBD protein is >80% pure (see Figure 2).
Citations	Malilas et al., 2013. Cancer upregulated gene 2, a novel oncogene, confers resistance to oncolytic vesicular stomatitis virus through STAT1-OASL2 signaling. Cancer Gene Ther. doi:10.1038/cgt.2012.96. Takeishi et al., 2012. Diacylglycerol kinase alpha enhances hepatocellular carcinoma progression by activation of Ras&ndash,Raf&ndash,MEK&ndash,ERK pathway. J. Hepatol. 57, 77-83. Zhang et al., 2004. Protein kinase C (PKC) &beta,II induces cell invasion through a Ras/Mek-, PKC &iota,/Rac 1-dependent signaling pathway. J. Biol. Chem. 279, 22118-22123.
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Alias	Small G protein, Raf, Raf-RBD, ras binding domain, c-Raf
Similar products	c-Raf, Raf, Small G protein, Raf-RBD, ras binding domain
Shipping condition	Room temperature
Available
Shipping Temperature	AT
Storage Conditions	On Arrival: 4°C
Delivery Time	1-2 Weeks
Weight (grams)	18
Product Uses	Measurement of the GTP/GDP ratio of Ras in vitro. Quantitation of GTP-Ras from tissue and tissue culture cell lysates.
Material	The Ras binding domain (RBD) of the c-Raf kinase protein (Raf) protein has been expressed as a GST-fusion protein in E. coli. This protein binds binds specifically to GTP-bound, and not GDP-bound, Ras proteins. The domain can therefore be used to specifically precipitate active, GTP-bound Ras as well as to specifically block the activity of Ras in vitro and in vivo. The protein is supplied in a glutathione agarose bound format and is shipped lyophilized. The beads are colored for ease of use. This product is used in our Ras activation assay Biochem Kit (Cat. # BK008). The GST-tagged Raf-RBD protein can be released from the beads by incubation with 10 mM reduced glutathione.
Biological Activity	Raf-RBD protein specifically recognizes and binds the active, GTP-bound, forms of Ras proteins. It has a much lower affinity for the inactive, GDP-bound, form Ras.When coupled to a colored glutathione sepharose matrix, the Raf-RBD protein beads become a convienent tool for assaying the activity of the Ras proteins. The quality control biological assay for Raf-RBD protein beads consists of a Ras protein pulldown frombovine brain extracts loaded with either GTP?S (Cat. # BS01) or GDP.
Figure 1 Legend	The brightly colored glutatione agarose beads in RF02 are easy to use.
Figure 2 Legend	Figure 2. GST-Raf-RBD protein purity determination. A 20 ug sample of RF02 was separated by electrophoresis in a 12% SDS-PAGE system and stained with Coomassie Blue. The GST-Raf-RBD protein runs at approximately 35 kDa.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

RF02-B.pdf

RF02-B-datasheet.pdf

RF02-B-safety-datasheet.pdf