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Rac1 His Protein: wild type

Rac1 His Protein: wild type

Item no.	RC01-A
Manufacturer	Cytoskeleton
Amount	1 x 100 ug
Quantity options	1 x 100 ug 3 x 100 ug 1 x 1 mg
Category	Peptides & Proteins Recombinant Proteins Cell Biology Cytoskeleton small GTPases Rho Subfamily
Type	Proteins
Specific against	other
Purity	Purity is determined by scanning densitometry of proteins on SDS-PAGE gels. His-Rac1 samples are >90% pure.
Citations	Kulkarni, S., Goll, D. E. and Fox, J. E. (2002). Calpain cleaves RhoA generating a dominant-negative form that inhibits integrin-induced actin filament assembly and cell spreading. J. Biol. Chem. 277, 24435-24441.
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Alias	Small G protein, Rac, Rac1, Rac 1, Rac1 protein
Similar products	Rac, Rac1, Rac 1, Small G protein, Rac1 protein
Shipping condition	Room temperature
Available
Shipping Temperature	AT
Storage Conditions	On Arrival: 4°C
Delivery Time	1-2 Weeks
Weight (grams)	7
Product Uses	Rac biochemistry Rac1 GTPase assays Rac1 nucleotide exchange assays Rac1 binding studies
Material	The human Rac1 protein has been produced in a bacterial expression system. The protein is supplied as a lyophilized powder.When it is reconstituted in distilled water to 5 mg/ml, the protein is in the following buffer: 50 mM Tris pH 7.5, 0.5 mM MgCl₂ 50 mM NaCl, 0.5% sucrose, and 0.1% dextran. Protein concentration is determined by the Precision Red Advanced Protein Assay Reagent, Cat. # ADV02. The recombinant protein is 22 kDa, consisting of the Rac1 protein plus a histidine tag in the amino-terminus. For other forms of Rac1 as well as many other purified small G-proteins, see our main small G-protein product page
Biological Activity	The biological activity of RC01 is determined by its ability to exchange nucleotide. This is tested by a pulldown assay using GST-tagged PAK-PBD beads (Cat. # PAK02) and GTP?S (Cat. # BS01) or GDP loaded His-Rac1. The PAK protein is an effector of Rac1, and will specifically bind to the GTP bound form Rac1. Using this assay, the amount of biologically active GTP-bound Rac1 is determined. Stringent quality control ensures that > 80% of the His-Rac1 protein produced is capable of binding GTP.
Figure 1 Legend	Figure 1. His-Rac1 protein purity determination. A 10 ug sample of RC01 (His-Rac1 molecular weight approx. 22 kDa) was separated by electrophoresis in a 12% SDS-PAGE system. The protein was stained with Coomassie Blue.<

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

RC01-A.pdf

RC01-A-datasheet.pdf

RC01-A-safety-datasheet.pdf