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Heavy Meromyosin Protein (Rabbit skeletal muscle)

Heavy Meromyosin Protein (Rabbit skeletal muscle)

Item no.	MH01-A
Manufacturer	Cytoskeleton
Amount	4 x 50 ug
Category	Neuroscience Neural Lineage Markers Molecular Targets for Neuroscience Peptides & Proteins Recombinant Proteins Cell Biology Cell Status Necrosis Cytoskeleton Motor proteins
Type	Proteins
Specific against	other
Purity	Protein purity is determined by scanning densitometry of Coomassie Blue stained protein on a 4-20% gradient polyacrylamide gel. Heavy meromyosin protein was determined to be 70% pure (see Figure 2).
Citations	Li, Y., Lin, J. L., Reiter, R. S., Daniels, K., Soll, D. R. and Lin, J. J. (2004). Caldesmon mutant defective in Ca2+-calmodulin binding interferes with assembly of stress fibers and affects cell morphology, growth and motility. J. Cell Sci. 117, 3593-3604. Kontrogianni-Konstantopoulos, A., Huang, S. C. and Benz, E. J., Jr. (2000). A nonerythroid isoform of protein 4.1R interacts with components of the contractile apparatus in skeletal myofibers. Mol. Biol. Cell 11, 3805-3817.
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Alias	Heavy meromyosin protein, meromyosin, meromyosin ATPase, meromyosin F-actin
Similar products	Heavy meromyosin protein, meromyosin, meromyosin ATPase, meromyosin F-actin
Shipping condition	Room temperature
Available
Shipping Temperature	AT
Storage Conditions	On Arrival: 4°C
Delivery Time	1-2 Weeks
Weight (grams)	26
Product Uses	Measurement of F-actin activated ATPase activity Identification/characterization of proteins or small molecules that affect heavy meromyosin ATPase activity Identification/characterization of proteins or small molecules that affect heavy meromyosin Factin interaction
Material	Heavy meromyosin has been produced by ?-chymotrypsin proteolytic cleavage of full-length myosin II protein isolated from rabbit skeletal muscle. Heavy meromyosin consists of an active motor fragment consisting of two head domains connected by their subfragment-2 (S2) regions and two pairs of light chains, essential light chain (ELC) and regulatory light chain (RLC), see Figure 1. Heavy meromyosin has been determined to be biologically active in an F-actin activated ATPase assay. Heavy meromyosin is supplied as a white lyophilized powder.When reconstituted in nanopure water, the protein will be in the following buffer: 10 mM Imidizole pH 7.0, 50 mM KCl, 3 mM MgCl₂ 1 mM DTT, 5% sucrose and 1% dextran. The lyophilized protein is stable at 4C desiccated (< 10% humidity) for 1 year.
Biological Activity	The biological activity of rabbit heavy meromyosin is determined from its rate of F-actin activated ATP hydrolysis. A standard biological assay for monitoring ATP hydrolysis by heavy meromyosin consists of an in vitro F-actin ATPase assay (Cat. # BK054). Stringent quality control ensures that in the presence of F-actin, rabbit heavy meromyosin will have a minimum hydrolysis rate 500 fold greater than in the absence of F-actin.
Figure 1 Legend	Figure 1. Diagrammatic representation of the myosin II protein and its subfragments. Myosin II or conventional myosin is a hexameric protein consisting of two heavy chains and two light chains. Myosin II can be proteolytically cleaved into heavy meromyosin (HMM) and light meromyosin(LMM) by ?-chymotrypsin. Heavy meromyosin consists of the myosin head subfragment-1 domain (S1), its associated light chains (essential light chains and regulatory light chains), and the coiled-coil subfragment -2 domain. Light meromyosin consists of coiled-coil protein structure. The myosin S1-subfragment is produced by papain digestion of HMM.
Figure 2 Legend	Figure 2. Heavy meromyosin purity determination. A 10 ug sample of MH01 was separated by electrophoresis in a 4-20% SDS-PAGE system, and stained with Coomassie Blue. Arrow indicates the truncated myosin head domain (approx. 150 kDa), arrowheads indicate the RLC (approx. 20 kDa) and ELC (approx. 17 kDa). Protein quantitation was performed using the Precision Red Protein Assay Reagent (Cat. #ADV02).

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

MH01-A.pdf

MH01-A-datasheet.pdf

MH01-A-safety-datasheet.pdf