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GGA3-PBD Arf Affinity Beads

GGA3-PBD Arf Affinity Beads

Item no.	GGA05-A
Manufacturer	Cytoskeleton
Amount	1 x 500 ug
Category	Oncology By Cell Type Blastoma Carcinoma Glioma Hematology Leukemia Lymphoma Myeloma Melanoma Sarcoma Cell Biology Cell Status Mesothelioma Senescence Reagents & Chemicals Nanoparticles Beads & Resins By Organ Lung Cancer
Type	Proteins
Specific against	other
Purity	A 20 ug sample of GGA3-PBD protein from two separate lots (molecular weight approx. 60 kDa) was separated by electrophoresis in a 4-20% SDS polyacrylamide gel, and stained with Coomassie Blue. Protein quantitation was performed using the Precision Red Pro
Citations	Takatsu, H., Yoshino, K., Toda, K., and Nakayama, K. (2002) GGA proteins associate with Golgi membranes through interaction between their GGAH domains and ADPribosylation factors.Biochem. J.365, 369-378.
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Alias	GGA3, GGA3-PBD, GGA3-Beads, Arf1, Arf6
Similar products	GGA3, Arf1, Arf6, GGA3-PBD, GGA3-Beads
Available
Shipping Temperature	AT
Storage Conditions	On Arrival: 4°C
Delivery Time	1-2 Weeks
Material	The GGA3-PBD containing residues 1-316 of GGA3 has been overexpressed in a bacterial expression system. The recombinant protein has a molecular weight of approximately 60kDa and contains a GST-tag at its amino terminus. The purified protein has been conjugated to colored sepharose beads and is supplied as a lyophilized purple powder (Figure 1). One tube of GGA07 should be sufficient for approximately 20-25 assays (see Biologcial Activity Assay on the product datasheet). The GGA3-PBD protein used for conjugation to the beads was analysed by scanning densitometry of a Coomassie Blue stained 4-20% SDS polyacrylamide gel and determined to be >80% pure (see Figure 2) The protein is supplied in a glutathione agarose bound format and is shipped lyophilized. The beads are colored for ease of use. This product is used in the Arf1 pull-down activation assay Biochem Kit (Cat. # BK032-S) and the Arf6 pull-down activation assay Biochem Kit (Cat. # BK033-S).
Biological Activity	The GGA3-PBD protein specifically recognizes and binds the active "GTP-bound" forms of the Arf1 and Arf6 proteins (1). It has a much lower affinity for the inactive "GDP-bound" forms of Arf1 and Arf6.When covalently coupled to a colored sepharose matrix, the GGA3-PBD beads become a convenient tool for assaying the activity of Arf1 and Arf6 proteins. For standard biological assays using the beads, see product datasheet.
Figure 2 Legend	Figure 2. A 20 ug sample of GGA3-PBD protein from two different lots (molecular weight approx. 60 kDa) was separated by electrophoresis in a 4-20% SDS polyacrylamide gel, and stained with Coomassie Blue. Protein quantitation was performed using the Precision Red Protein Assay Reagent (Cat. # ADV02). See Blue molecular weight markers are from Invitrogen.

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All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.