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Dbs protein GEF domain - HIS fusion

Dbs protein GEF domain - HIS fusion

Item no.	GE01-A
Manufacturer	Cytoskeleton
Amount	2 x 50 ug
Category	Neuroscience Neural Signaling Peptides & Proteins Recombinant Proteins Cell Biology Cytoskeleton small GTPases Rho Subfamily
Type	Proteins
Specific against	other
Purity	Purity is determined by scanning densitometry of proteins run on SDS-PAGE gels. Samples are >80% pure.
Citations	H. Jeon et al. (2011). Phospholipase D2 induces stress fiber formation through mediating nucleotide exchange for RhoA. Cellular Signalling. v 23, pp 1320-1326.
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Alias	Small G protein, GEF activity, Dbs binding proteins, Dbs, His-Dbs, RhoGEF positive control, RhoGEF
Similar products	RhoGEF, Small G protein, GEF activity, Dbs binding proteins, Dbs, His-Dbs, RhoGEF positive control
Shipping condition	Room temperature
Available
Shipping Temperature	AT
Storage Conditions	On Arrival: 4°C
Delivery Time	1-2 Weeks
Weight (grams)	9
Product Uses	Study of GEF activity with different GTPases. Identification of Dbs binding proteins Study of Dbs function in vivo by the introduction of His-Dbs into live cells Postive control for other RhoGEF studies.
Material	The Guanine nucleotide Exchange Factor domain (GEF domain, also known as the DH/PH tandem domain) of the human RhoGEF Dbs (hDbs) has been produced in a bacterial expression system with a 6xHis tag at its amino terminus. Dbs is an efficient GEF for the RhoA and Cdc42 GTPases and it shows weak GEF activity towards Rac1. The molecular weight of this His-hDbs DH/PH protein is approximately 40 kDa. The protein is supplied as a lyophilized powder.When reconstituted to 2 mg/ml, the protein is in the following buffer: 20 mM Tris pH 7.5, 0.5 mM MgCl₂ 0.5% sucrose and 0.1% dextran. Protein concentration is determined by the Precision Red Advanced Protein Assay Reagent (Cat. # ADV02)
Biological Activity	The biological activity of the hDbs DH/PH was tested by measuring the ablility of the protein to catalyze the exchange of GDP for mant-GTP on Cdc42 and RhoA (Cat. # CD01 and RH01 respectively) using the RhoGEF exchange assay kit (Cat. # BK100). See Figure 2.
Figure 1 Legend	Figure 1. His-hDbs DH/PH protein purity determination. A 10 ug sample of GE01 was separated by electrophoresis in a 12% SDS-PAGE system. The protein was stained with Coomassie Blue.
Figure 2 Legend	hDbs DH/PH stimulates nuleotide exchange on Cdc42 as determined by BK100

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

GE01-A.pdf

GE01-A-datasheet.pdf

GE01-A-safety-datasheet.pdf