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Cdc42-His Protein: Wild type

Cdc42-His Protein: Wild type

Item no.	CD01-XL
Manufacturer	Cytoskeleton
Amount	1 x 1 mg
Quantity options	1 x 100 ug 3 x 100 ug 1 x 1 mg
Category	Metabolism Diabetes Cholesterol & Lipid Metabolism Regulation of Appetite Peptides & Proteins Recombinant Proteins Cell Biology Cytoskeleton small GTPases Rho Subfamily
Type	Proteins
Specific against	other
Purity	Purity is determined by scanning densitometry of proteins on SDS-PAGE gels. His-Cdc42 samples are >90% pure.
Citations	Nevins, A. K. and Thurmond, D. C. (2005). A direct interaction between Cdc42 and vesicle-associated membrane protein 2 regulates SNARE-dependent insulin exocytosis. J. Biol. Chem. 280, 1944-1952. Kulkarni, S., Goll, D. E. and Fox, J. E. (2002). Calpain cleaves RhoA generating a dominant-negative form that inhibits integrin-induced actin filament assembly and cell spreading. J. Biol. Chem. 277, 24435-24441.
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Alias	Small G protein, Cdc42 protein, Cdc42, Cdc 42, Cdc42 GTPase assay, his-Cdc42
Similar products	Cdc42, Cdc 42, Small G protein, Cdc42 protein, his-Cdc42, Cdc42 GTPase assay
Shipping condition	Room temperature
Available
Shipping Temperature	AT
Storage Conditions	On Arrival: 4°C
Delivery Time	1-2 Weeks
FAQs	Question 1: After reconstituting the lyophilized protein with water, what is the composition of the buffer the wild-type human Cdc42 protein is in? Answer 1: The protein should be reconstituted to 5 mg/ml by the addition of Milli-Q water. The protein will be in the following buffer: 50 mM Tris pH 7.6, 0.5 mM MgCl₂, 50 mM NaCl, 3% sucrose, and 0.6% dextran. In order to maintain high biological activity of the protein, it is strongly recommended that the protein solution be supplemented with DTT to 1 mM final concentration. Question 2: Can the wild-type human Cdc42 protein be microinjected into cells? Answer 2: Yes, the wild-type human Cdc42 protein can be microinjected into living cells to monitor the localization of Cdc42.
Weight (grams)	80
Product Uses	Cdc42 biochemistry Cdc42 GTPase assays Cdc42 nucleotide exchange assays Cdc42 binding studies
Material	The human Cdc42 protein has been produced in a bacterial expression system. The protein is supplied as a lyophilized powder.When it is reconstituted in distilled water to 1 mg/ml, the protein is in the following buffer: 2 mM Tris pH 7.6, 0.5 mM MgCl₂ 0.5% sucrose, 0.1% dextran. Protein concentration is determined by the Precision Red Advanced Protein Assay Reagent, Cat. # ADV02. The recombinant protein is 22 kDa, consisting of the 22 kDa Cdc42 protein plus a 6 amino acid histidine tag in the amino-terminus. For other forms of Cdc42 as well as many other purified small G-proteins, see our main small G-protein product page
Biological Activity	The biological activity of CD01 is determined by its ability to exchange nucleotide. This is tested by a pulldown assay using GST-tagged PAK-1 PBD beads (Cat. # PAK02) and GTP?S (Cat. # BS01) or GDP loaded His-Cdc42. The PAK (p21 activated kinase) protein is an effector of Cdc42, and will specifically bind to the GTP bound form Cdc42. Using this assay, the amount of biologically active GTP-bound Cdc42 is determined. Stringent quality control ensures that > 70% of the Cdc42 protein produced is capable of binding GTP.
Figure 1 Legend	His-Cdc42 protein purity determination. A 10 ug sample of CD01 (His-Cdc42 molecular weight approx. 22 kDa) was separated by electrophoresis in a 12% SDS-PAGE system. The protein was stained with Coomassie Blue.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

CD01-XL.pdf

CD01-XL-datasheet.pdf

CD01-XL-safety-datasheet.pdf