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GTP gamma S

GTP gamma S

Item no.	BS01
Manufacturer	Cytoskeleton
Amount	50 ml (20 mM stock)
Category	Peptides & Proteins Recombinant Proteins Cell Biology Cytoskeleton small GTPases Rho Subfamily
Type	Proteins
Specific against	other
Purity	Purity was determined by HPLC. The product is >75% pure.
Citations	Coming soon! For the most recent publications citing this product, please contact our Technical Service department at infohoelzel.de
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Alias	Small G protein, Guanosine gamma thio-phosphate, GTPyS, buffer, inhibitor
Similar products	inhibitor, Small G protein, Guanosine gamma thio-phosphate, GTPyS, buffer
Shipping condition	Room temperature
Available
Shipping Temperature	AT
Storage Conditions	On Arrival: 4°C
Delivery Time	1-2 Weeks
FAQs	Question 1: Can binding of GTP&gamma, S to Ras, Arf, and Rho family GTPases be detected with effector proteins conjugated to Sepharose beads? Answer 1: Yes, GTP&gamma, S can be used to create activated Ras, Arf, and Rho family GTPases which can be pulled down with Sepharose beads conjugated to an effector protein specific for the GTPase of interest. The GTP&gamma, S should give greater than five-fold increase in activated small G-protein when compared to a GDP bound protein. Question 2: What percentage of GTPase in an extract will bind to the GTP&gamma, S and so constitute an activated small G-protein? Answer 2: Approximately 5-10% of the total population of Ras, Arf, or Rho family GTPase will bind to the GTP&gamma, S, creating an activated GTPase protein.
Weight (grams)	9
Product Uses	Create constitutively GTP bound GTPases Activate small G-proteins
Material	Guanosine gamma thio-phosphate (GTP?S), a non-hydrolyzable GTP analog. Upon resuspension with 50 ul de-ionized water you will have a 20 mM stock which is enough for 50 assays of small G-protein activation.
Biological Activity	Biological activity was determined by the ability of the compound to activate RhoA-His protein (Cat. # RH01). Greater than 90% activation is achieved at a concentration of 200 uM BS01.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

BS01.pdf

BS01-datasheet.pdf

BS01-safety-datasheet.pdf