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Fibronectin Rhodamine (bovine plasma)

Fibronectin Rhodamine (bovine plasma)

Item no.	FNR01-A
Manufacturer	Cytoskeleton
Amount	5 x 20 ug
Quantity options	5 x 20 ug 20 x 20 ug Large Quantities
Category	Oncology Neuroscience Neural Adhesion Molecules By Cell Type Peptides & Proteins Recombinant Proteins Cell Biology Cell Movement Cell Adhesion Cytoskeleton Cell Stainings ECM Microfilaments small GTPases Rho Subfamily
Type	Proteins
Specific against	Cattle (Bovine)
Purity	Purity is determined by scanning densitometry of proteins on SDS-PAGE gels. Samples are >90% pure. No free dye is apparent in the final product.
Citations	Steele et al., 2012. Tandem zyxin LIM sequences do not enhance force sensitive accumulation. Biochem. Biophys. Res. Commun. v 422, pp 653&ndash,657. Nakayama et al., 2012. Thermoresponsive Poly(N-isopropylacrylamide)-Based Block Copolymer Coating for Optimizing Cell Sheet Fabrication. Macromol. Biosci. v 12, pp 751&ndash,760. Tamura et al., 2012. Thermally responsive microcarriers with optimal poly(N-isopropylacrylamide) grafted density for facilitating cell adhesion/detachment in suspension culture. Acta Biomaterialia. doi:http://dx.doi.org/10.1016/j.actbio.2012.07.006. Steward et al., 2011. Mechanical stretch and shear flow induced reorganization and recruitment of fibronectin in fibroblasts. Sci. Rep. 1, p 147. doi: 10.1038/srep00147. Nagase et al., 2011. Thermo-Responsive Polymer Brushes as Intelligent Biointerfaces: Preparation via ATRP and Characterization. Macromol Biosci. v 11, pp 300-309. Robinson et al., 2004. Fibronectin matrix assembly regulates &alpha,5&beta,1-mediated cell cohesion. Mol. Biol. Cell. v 15, pp 973-981. Brock et al., 2003. Geometric determinants of directional cell motility revealed using microcontact printing. Langmuir. v 19, pp 1611-1617.
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Alias	Actin, Actin Protein, rhodamine fibronectin, fibronectin matrix, Fibronectin,
Similar products	Fibronectin, Actin, Actin Protein, rhodamine fibronectin, fibronectin matrix
Shipping condition	Room temperature
Available
Shipping Temperature	AT
Storage Conditions	On Arrival: 4°C
Delivery Time	1-2 Weeks
FAQs	Question 1: What is the optimal excitation and emission filter settings to visualize the rhodamine fluorescence? Answer 1: Rhodamine fibronectin can be detected using a filter set of 535 nm excitation and 585 nm emission. Question 2: What is the labeling stoichiometry? Answer 2: Rhodamine labeling stoichiometry was calculated to be 1-3 dyes per fibronectin protein using the absorbance maximum for rhodamine at 565 nm and the Beer-Lambert law. Dye extinction coefficient when protein bound is 70, 000 M^-1cm^-1.
Weight (grams)	15
Product Uses	Tracking cell movement through a 3D matrix Tracking cell movement across a glass slide Observation of fibronectin matrix assembly and cell adhesion. FACS analysis of fibronectin binding cells
Material	Fibronectin purified from bovine plasma and has been modified to contain a covalently linked rhodamine fluorescent dye. An activated ester of rhodamine has been used to label the protein with a labeling stoichiometry of apprximately 1.0 dyes per protein molecule, a low labeling stiochiometry to retain functional activity. No free dye is apparent in the final product. Fibronectin has an approximate molecular weight of 250 kDa. FNR01 (20 ug of protein) is provided as a lyophilized powder. Fluorescent Fibronectin Treated MCF10A cells Fluorescent fibronectin (Cat. # FNR01) treated MCF10Acells (image kindly provided by A. Varadara and M. Karthykenyan, Univ. S.Carolina, Columbia, SC).
Figure 1 Legend	Figure 1: Rhodamine Fibronectin Purity Determination. A 20 ug sample of rhodamine fibronectin (molecular weight approx. 250 kDa) was separated by electrophoresis oin a 4-20% SDS-PAGE system and stained with Coomassie Blue.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

FNR01-A.pdf

FNR01-A-datasheet.pdf

FNR01-A-safety-datasheet.pdf