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Actin protein (bovine cardiac muscle, >99% pure)

Actin protein (bovine cardiac muscle, >99% pure)

Item no.	AD99-A
Manufacturer	Cytoskeleton
Amount	1 x 1 mg
Quantity options	1 x 1 mg 5 x 1 mg
Category	Peptides & Proteins Recombinant Proteins Cell Biology Cytoskeleton Microfilaments
Type	Proteins
Specific against	Cattle (Bovine)
Purity	Purity is determined by scanning densitometry of proteins on SDS-PAGE gels. Samples are > 99% pure.<br /><strong>Biological Activity:</strong><br />The biological activity is determined in three ways. The first method utilizes pyrene muscle actin as a
Citations	Butler S.C., et al. 2012. Inhibitory effects of pectenotoxins from marine algae on the polymerization of various actin isoforms. Toxicol. In Vitro. 26, 493-499. Fressinaud C., et al. 2012. Axon cytoskeleton proteins specifically modulate oligodendrocyte growth and differentiation in vitro. Neurochem. Int. 60, 78-90. Orlova A., et al. 2011. The N-terminal domains of myosin binding protein C can bind polymorphically to F-actin. J. Mol. Cell. 412, 379&ndash,386. Debold E.P., et al. 2010. Human actin mutations associated with hypertrophic and dilated cardiomyopathies demonstrate distinct thin filament regulatory properties in vitro. J. Mol. Cell. Cardiol. 48, 286-292. Passarelli C., et al. 2010. Susceptibility of isolated myofibrils to in vitro glutathionylation: Potential eelevance to muscle functions. Cytoskeleton. 67, 81&ndash,89.
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Alias	Actin, Actin Protein, cardiac muscle, cardiac actin, cardiac muscle actin, actin cardiac muscle
Similar products	Actin, cardiac muscle, Actin Protein, cardiac actin, cardiac muscle actin, actin cardiac muscle
Shipping condition	Room temperature
Available
Shipping Temperature	AT
Storage Conditions	On Arrival: 4°C
Delivery Time	1-2 Weeks
FAQs	Question 1: What is the isotype composition of the cardiac actin from bovine heart muscle? Answer 1: Bovine cardiac actin ((Cat. #AD99) is composed of 84% a-cardiac and 16% a??˜-skeletal actin isoforms. Question 2: How do I measure actin polymerization with the pyrene assay when there is no available pyrene conjugate for cardiac actin? Answer 2: To examine the polymerization of unlabeled cardiac actin ((Cat. #AD99), please click here for a polymerization protocol that uses an excess of the unlabeled [cardiac] actin plus 10% of pyrene-labeled skeletal muscle actin. The pyrene muscle actin will not polymerize on its own at the concentration used in this assay, so the reaction is dependent on unlabeled cardiac actin ((Cat. #AD99) for F-actin formation. In this way, the pyrene-labeled muscle actin is taken up and polymerized to serve as a reporter for polymerization of the unlabeled actin that is present at a much greater concentration.
Weight (grams)	7
Product Uses	Identification and characterization of cardiac actin binding proteins In vitro cardiac actin polymerization studies
Material	Bovine cardiac muscle actin is supplied as a lyophilized powder.When one vial is reconstituted in 1 ml of Actin Monomer Buffer: 5.0 mM Tris-HCI pH 8.0, 0.2 mM CaCl₂ 0.2 mM ATP and 0.5 mM DTT, it is at a final concentration of 1.0 mg/ml.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

AD99-A.pdf

AD99-A-datasheet.pdf

AD99-A-safety-datasheet.pdf