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Signal-Seeker™ Phosphotyrosine Detection Kit (30 assays)

Signal-Seeker™ Phosphotyrosine Detection Kit (30 assays)

Item no.	BK160
Manufacturer	Cytoskeleton
Amount	KIT 30 assays
Category	Other Assays & Kits Other Kits
Type	Assay
Specific against	other
ECLASS 10.1	32161000
ECLASS 11.0	32161000
UNSPSC	41116126
Available
Advantages	Discover and publish novel regulatory mechanisms. Detect highly transient regulation of protein modifications. Use different kits to build a temporal protein regulation profile. Investigate the role of known protein modifications in your system. Obtain data for endogenous proteins. Discover novel biomarkers. Complements and enriches proteomic approaches.
Associated Products	Signal Seeker Ubiquitination Enrichment Kit (Cat. # BK161) Anti-Phosphotyrosine HRP conjugated monoclonal antibody (Cat.# APY03-HRP) Anti-Phosphotyrosine Affinity Beads (Cat.# APY03-beads) Rac Activator I (Cat.# CN02)
Background	Signal Seeker kits use affinity beads to pull-out and enrich modified proteins from any given cell or tissue lysate. The enriched protein population is then analyzed by standard western blot procedures using a primary antibody to the target protein.
Kit contents	The kit contains sufficient reagents to perform 30 phosphotyrosine immunoprecipitation reactions: The following components are included in the kit. BLASTR Lysis Buffer BLASTR Dilution Buffer BLASTR Wash Buffer Protease Inhibitor Cocktail Tyrosine Phosphatase Inhibitor Phosphotyrosine Affinity Beads IP Control Beads Anti-phosphotyrosine-HRP antibody Precision Red Protein Assay Reagent Bead Elution Buffer DMSO Chemiluminescent Reagent A Chemiluminescent Reagent B Spin Columns
Overview	Protein regulation during signal transduction and other cellular events is, by necessity, a rapid and dynamic process. Most often, these mechanisms involve modification of an extremely small fraction of the protein target. This makes the scientists job of capturing key regulatory processes difficult and frustrating. Analysis of key regulatory mechanisms involving phosphorylation, ubiquitination, SUMOylation or acetylation has historically required a huge investment of time and effort.
Shipping Temp.	AT
Storage on Arrival	4C

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: KIT 30 assays

available

Delivery expected until 12/13/2024