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Chemically Acetylated Bovine Serum Albumin (BSA)

Chemically Acetylated Bovine Serum Albumin (BSA)

Item no.	AACX1
Manufacturer	Cytoskeleton
Amount	2 x 500 ug
Category	Peptides & Proteins Recombinant Proteins
Type	Proteins
Specific against	other
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Shipping condition	Room temperature
Available
Shipping Temperature	AT
Storage Conditions	On Arrival: 4°C
Weight (grams)	10
Product Uses	Positive control for anti-acetyl antibody (Cat. #AAC01) specificity and sensitivity (Fig.1). Competitive inhibitor for antibody binding to acetyl lysine targets (Fig. 2)
Material	Lysine residues of bovine serum albumin (BSA) have been chemically acetylated by acetic anhydride under basic conditions. A major Ac-BSA band runs at approximately 66 KDa and Ac-BSA oligos run at approximately 120-260 KDa. NOTE: Ac-BSA oligomerization is not caused by the acetylation reaction as these species are also present in unmodified BSA as seen in a Coomassie stain of unmodified BSA protein. Acetylated BSA is supplied as a lyophilized powder.
Figure 1 Legend	Legend: Lanes 1-5, 0.5, 0.1, 0.05, 0.01 and 0.005 ng Ac-BSA respectively. Lanes 6 & 7, 500 and 1000 ng of non-acetylated BSA. Western blots of Ac-BSA and unmodified BSA were ana-lysed usingAAC01 anti-acetyl antibody. The 1000 ng of non-acetylated BSA did not produce a signal while Ac-BSA was de-tected down to 0.005 ng. The major BSA band runs at approxi-mately 66KD (arrowhead) with BSA oligomers running between approximately 120-260 KDa.
Figure 2 Legend	Legend: Lysates were made from A431 cells that were treated (+) or untreated (-) with the HDAC inhibitor trichostatin A (TSA). Each lane represents 20 ug of cell lysate. Lysates were run on SDS-PAGE and transferred to PVDF membranes. Membranes were probed with anti-acetyl lysine antibody (Cat. # AAC01) at 1:500 dilution in TBST in the presence of 10 ug/ml BSA or 10 ug/ml of Ac-BSA. The Ac-BSA eliminates all acetyl lysine signal on the membrane. Prominent bands in the TSA treated lanes represent acetyl tubulin (55 KDa) and acetyl histones (14-16 KDa).

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

AACX1.pdf

AACX1-datasheet.pdf

AACX1-safety-datasheet.pdf