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General Tubulin Buffer

General Tubulin Buffer

Item no.	BST01-001
Manufacturer	Cytoskeleton
Amount	1 x 10 ml
Quantity options	1 x 10 ml 1 x 100 ml 10 x 100 ml
Category	Oncology By Cell Type Carcinoma Hematology Myeloma Cell Biology Cell Status Cytoskeleton Autophagy Reagents & Chemicals Biochemicals Buffers Mikrotubules By Organ Breast Cancer Testicular & Prostate Cancer
Type	Buffer
Specific against	other
Citations	Coming soon! For the most recent publications citing this product, please contact our Technical Service department at infohoelzel.de
ECLASS 10.1	42051003
ECLASS 11.0	42051003
UNSPSC	12161700
Alias	tubulin, General tubulin buffer, General tubulin, tubulin buffer, PEM
Similar products	PEM, tubulin, General tubulin buffer, General tubulin, tubulin buffer
Shipping condition	Room temperature
Available
Shipping Temperature	AT
Storage Conditions	On Arrival: 4°C
Delivery Time	1-2 Weeks
FAQs	Question 1: How does general tubulin buffer differ from G-PEM buffer? Answer 1: Basically, these buffers are the same except that G-PEM contains 1mM GTP to support tubulin polymerization. General tubulin buffer ((Cat. #BST01) and PEM buffer typically contain 80 mM PIPES, 2 mM MgCl2, 0.5 mM EGTA pH 7.0. Both buffers should be supplemented with 1 mM GTP (hence, the &ldquo, G&rdquo, in G-PEM buffer). Question 2: Is glycerol necessary for the maintenance of biologically-active tubulin? Answer 2: Glycerol is often added to a final concentration of 5 - 10% to enhance polymerization, however, glycerol is not necessary for the maintenance of biologically active tubulin.
Weight (grams)	19
Product Uses	Resuspension and storage of tubulin
Material	General tubulin buffer (PEM).Contains 80 mM PIPES pH 6.9, 2 mM MgCl₂ and 0.5 mM EGTA. Used as a tubulin working buffer in the form of G-PEM (i.e. PEM plus 1 mM GTP (Cat.# BST06)) or a tubulin polymerization buffer in the form of glycerol containing G-PEM (PEM plus 1 mM GTP (Cat. # BST06) and e.g. 5% glycerol (see Cat. # BST05)).

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

BST01-001.pdf

BST01-001-datasheet.pdf

BST01-001-safety-datasheet.pdf