Comparison

Rac1 G-LISA Activation Assay Kit (colorimetric)

Item no. BK128-S
Manufacturer Cytoskeleton
Amount KIT 24 assays
Quantity options KIT 96 assays KIT 24 assays
Category
Type Assay
Specific against other
ECLASS 10.1 32161000
ECLASS 11.0 32161000
UNSPSC 41116126
Shipping condition Room temperature
Available
Shipping Temperature
AT
Storage Conditions
On Arrival: 4°C
Kit contents
-Enough reagents for 96 assays (Stripwell)
96 individual Rac1-GTP binding wells
Anti-Rac1 antibody (Cat. #ARC03)
Secondary Antibody - HRP
Rac1 control protein
Cell Lysis Buffer
Wash buffer
Antigen Presenting Buffer
Antibody Dilution Buffer
HRP Detection and Stop Reagents
Precision Red Advanced Protein Assay (Cat. #ADV02)
Protease Inhibitor Cocktail (Cat. #PIC02) Go to main G-LISA page
Description
The proprietary Rac1 G-LISA Activation Assaythat is faster, easier and more precise than traditional pull-down methods to measure Rac1 Activation.
Specific for Rac1
Colorimetric Based
Fast Results
Extremely Sensitive
General Information
This Rac1 G-LISA is a colorimetric based assay and shares the many advantages that all G-LISA activation assay have. This assay is specific for Rac1.
The Rac1 G-LISA kit contains a Rac-GTP-binding protein linked to the wells of a 96 well plate. Active GTP-bound Rac1 is captured by this protein while inactive GDP-bound Rac1 is removed following washes. The active Rac1 bound to the wells is detected with a Rac1 specific antibody. The degree of Rac1 activation is determined by comparing readings from activated cell lysates versus non-activated cell lysates.
Weight (grams)
600
Figure 1 Legend
Figure 1. The simple procedure of the Rac1 G-LISA assay.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: KIT 24 assays
Available: In stock
available

Compare

Add to wishlist

Get an offer

Request delivery time

Ask a technical question

Submit a bulk request

Questions about this Product?